Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms

被引：166
|
作者：
Andenmatten, Nicole [1 ]
Egarter, Saskia [1 ]
Jackson, Allison J. [1 ]
Jullien, Nicolas [2 ,3 ]
Herman, Jean-Paul [2 ,3 ]
Meissner, Markus [1 ]
机构：
[1] Univ Glasgow, Wellcome Trust Ctr Mol Parasitol, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow, Lanark, Scotland
[2] CNRS, Ctr Rech Neurobiol & Neurophysiol Marseille, Unite Mixte Rech 7286, Marseille, France
[3] Aix Marseille Univ, Marseille, France
来源：
NATURE METHODS | 2013年 / 10卷 / 02期
基金：
英国惠康基金;
关键词：
HOST-CELL INVASION;
D O I：
10.1038/NMETH.2301
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We established a conditional site-specific recombination system based on dimerizable Cre recombinase-mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncovered the existence of an alternative invasion pathway in apicomplexan parasites.
引用
收藏
页码：125 / 127
页数：3
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