Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms

被引：166

作者：

Andenmatten, Nicole ^{[1
]}

Egarter, Saskia ^{[1
]}

Jackson, Allison J. ^{[1
]}

Jullien, Nicolas ^{[2
,3
]}

Herman, Jean-Paul ^{[2
,3
]}

Meissner, Markus ^{[1
]}

机构：

[1] Univ Glasgow, Wellcome Trust Ctr Mol Parasitol, Inst Infect Immun & Inflammat, Coll Med Vet & Life Sci, Glasgow, Lanark, Scotland

[2] CNRS, Ctr Rech Neurobiol & Neurophysiol Marseille, Unite Mixte Rech 7286, Marseille, France

[3] Aix Marseille Univ, Marseille, France

来源：

NATURE METHODS | 2013年 / 10卷 / 02期

基金：

英国惠康基金;

关键词：

HOST-CELL INVASION;

D O I：

10.1038/NMETH.2301

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We established a conditional site-specific recombination system based on dimerizable Cre recombinase-mediated recombination in the apicomplexan parasite Toxoplasma gondii. Using a new single-vector strategy that allows ligand-dependent, efficient removal of a gene of interest, we generated three knockouts of apicomplexan genes considered essential for host-cell invasion. Our findings uncovered the existence of an alternative invasion pathway in apicomplexan parasites.

引用

页码：125 / 127

页数：3

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