Composition and Antimicrobial Activity of the Skin Peptidome of Russian Brown Frog Rana temporaria

被引：23

作者：

Samgina, T. Yu ^{[1
]}

Vorontsov, E. A. ^{[1
]}

Gorshkov, V. A. ^{[1
]}

Hakalehto, E. ^{[2
]}

Hanninen, O. ^{[3
]}

Zubarev, R. A. ^{[4
]}

Lebedev, A. T. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Organ Chem, Moscow, Russia

[2] Univ Eastern Finland, Dept Biosci, FI-70211 Kuopio, Finland

[3] Univ Eastern Finland, Dept Physiol, FI-70211 Kuopio, Finland

[4] Karolinska Inst, Div Mol Biometry, Dept Med Biochem & Biophys, Stockholm, Sweden

来源：

JOURNAL OF PROTEOME RESEARCH | 2012年 / 11卷 / 12期

关键词：

peptides sequencing mass spectrometry; HCD; ETD; Salmonella; Staphylococcus aureus; MRSA; antimicrobial frog peptides; antibiotics; PMEU; bacterial growth inhibition; BRADYKININ-RELATED PEPTIDES; HOST-DEFENSE PEPTIDES; GLANDULAR SECRETIONS; ENRICHMENT UNIT; IDENTIFICATION;

D O I：

10.1021/pr300890m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A nano-HPLC-ESI-OrbiTrap study involving HCD and ETD spectra has been carried out to clarify the composition of the skin peptidome of brown Russian frogs Rana temporaria. This approach allowed determinantion of 76 individual peptides, increasing 3-fold the identified portion of the peptidome in comparison to that obtained earlier with FTICR MS. A search for the new bradykinin related peptides (BRPs) was carried out by reconstructing mass chromatograms based on the ion current of characteristic b- and y-ions. Several peptides were reported in the secretion of R. temporaria for the first time. The overall antibacterial activity of the skin secretion in general and of one individual peptide (Brevinin 1Tb) was determined using PMEU Spectrion (Portable Microbe Enrichment Unit) technology. The inhibitory effects of these peptides on Staphylococcus aureus and Salmonella enterica Serovar typhimutium were equal in scale to that reported for some antibiotics.

引用

页码：6213 / 6222

页数：10

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