A Novel PCR Assay for Quantification of HIV-1 RNA

被引：72

作者：

Shan, Liang ^{[1
]}

Rabi, S. Alireza ^{[1
]}

Laird, Gregory M. ^{[1
]}

Eisele, Evelyn E. ^{[1
,2
]}

Zhang, Hao ^{[3
]}

Margolick, Joseph B. ^{[3
]}

Siliciano, Robert F. ^{[1
,4
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD USA

[4] Johns Hopkins Univ, Sch Med, Howard Hughes Med Inst, Baltimore, MD 21205 USA

来源：

JOURNAL OF VIROLOGY | 2013年 / 87卷 / 11期

关键词：

VIRUS-LIKE PARTICLES; ACTIVE ANTIRETROVIRAL THERAPY; TYPE-1; RNA; IN-VITRO; P-TEFB; IMMUNODEFICIENCY; PLASMA; INFECTION; TAT; VIREMIA;

D O I：

10.1128/JVI.00006-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This assay can detect and quantify HIV-1 RNA with high specificity and sensitivity.

引用

页码：6521 / 6525

页数：5

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