In situ detection of reovirus in formalin-fixed, paraffin-embedded chicken tissues using a digoxigenin-labeled cDNA probe

被引：11
|
作者：
Liu, HJ
Giambrone, JJ
机构：
[1] AUBURN UNIV, DEPT POULTRY SCI, AUBURN, AL 36849 USA
[2] AUBURN UNIV, ALABAMA AGR EXPT STN, AUBURN, AL 36849 USA
来源：
AVIAN DISEASES | 1997年 / 41卷 / 02期
关键词：
avian reovirus; in situ hybridization; digoxigenin-labeled cDNA probe;
D O I：
10.2307/1592203
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
An in situ hybridization (ISH) technique using a digoxigenin (DIG)-labeled cDNA probe detected avian reovirus (ARV) RNA in formalin-fixed, paraffin-embedded chicken tissues. Tissues were collected 3 and 10 days following inoculation with the R-2 or the S1133 strain of ARV. The cDNA clone HJp1, located on the S1 gene segment of the ARV S1133 strain, was used to prepare a nonradioactive probe. The ISH assay localized ARV RNA in infected tissues including heart, liver, intestine, pancreas, and tendon. No positive-stained cells occurred in sections from uninfected chickens.
引用
收藏
页码：447 / 451
页数：5
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