Assessment of genetic diversity of pigeonpea cultivars using RAPD analysis

被引：20
|
作者：
Ray Choudhury, P. [1 ]
Singh, I. P. [1 ]
George, B. [1 ]
Verma, A. K. [1 ]
Singh, N. P. [1 ]
机构：
[1] Indian Inst Pulses Res, Biotechnol Unit, Kanpur 208024, Uttar Pradesh, India
来源：
BIOLOGIA PLANTARUM | 2008年 / 52卷 / 04期
关键词：
DNA fingerprinting; molecular marker; DNA amplification; Cajanus cajan;
D O I：
10.1007/s10535-008-0126-0
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In our present study assessment of genetic diversity and identification of pigeonpea cultivars has been done by employing 76 random amplified polymorphic DNA (RAPD) primers. Out of 796 amplified products, 587 showed polymorphism (73.7 %) and an average of 10.47 bands were amplified per primer. Cluster analysis based on Jaccard's similarity coefficient using UPGMA grouped all the cultivars into three clusters. The cluster I consists of 7 cultivars, cluster II of 11 cultivars in 4 sub-clusters and cluster III 4 cultivars. Two cultivars were not included in any cluster. The clustering was strongly supported by high bootstrap values. Furthermore, high values of the average heterozygosity (H-av) and marker index (MI) also indicated the efficiency of RAPD as a marker system.
引用
收藏
页码：648 / 653
页数：6
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