Isolation of novel microsatellites from Jatropha curcas L. and their cross-species amplification

被引：15

作者：

Pamidimarri, D. V. N. Sudheer ^{[1
]}

Sinha, Ramini ^{[1
]}

Kothari, Pragya ^{[1
]}

Reddy, Muppala P. ^{[1
]}

机构：

[1] CSIR, Cent Salt & Marine Chem Res Inst, Discipline Wasteland Res, Bhavnagar 364002, Gujarat, India

来源：

MOLECULAR ECOLOGY RESOURCES | 2009年 / 9卷 / 01期

关键词：

biodiesel; cross-species amplification; FIASCO (fast isolation by AFLP of sequences containing repeats); Jatropha curcas; microsatellites;

D O I：

10.1111/j.1755-0998.2008.02471.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Jatropha curcas L., a member of the Euphorbiaceae, is widely distributed in different parts of the globe. In the present study, 12 microsatellites were isolated from J. curcas and their cross-species amplification was checked in six species of genus Jatropha. Within J. curcas, observed and expected heterozygosities ranged from 0.94 to 0.54 and from 0.95 to 0.56, respectively. Of the 12 loci, five showed significant deviation from Hardy-Weinberg equilibrium. There was no significant linkage disequilibrium detected between any of the loci. The markers isolated in the present investigation will be useful for assessing the population diversity and genetic structure of J. curcas and also in other species of Jatropha.

引用

页码：431 / 433

页数：3

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