Aggregation of LDL with chondroitin-4-sulfate makes LDL oxidizable in the presence of water-soluble antioxidants

The content of plasma and arterial interstitial fluid in water-soluble antioxidants makes it unlikely for low-density lipoprotein (LDL) to oxidize by the oxidation mechanisms most frequently discussed. By aggregation of LDL in the presence of chondroitin-4-sulfate (C-4-S), but not with chondroitin-6-sulfate or sphingomyelinase, a complex arises which can oxidize in the presence of 20 muM ascorbate and 300 muM urate. This oxidation sensitivity even persists after the gel-filtration of an LDL/C-4-S/ Cu2+ complex, indicating entrapment of Cu2+ within. This corresponds well to the known ability of C-4-S to bind copper ions and is a potential mechanism by which LDL oxidation in the arterial intima is facilitated after prolonged retention by the extracellular matrix. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.