All-solid-state lock-in imaging for wide-field fluorescence lifetime sensing

被引：43

作者：

Esposito, A

Oggier, T

Gerritsen, HC

Lustenberger, F

Wouters, FS

机构：

[1] European Neurosci Inst Gottingen, Cell Biophys Grp, D-37073 Gottingen, Germany

[2] Univ Utrecht, Debye Inst, NL-3508 TA Utrecht, Netherlands

[3] Ctr Suisse Elect & Microtech SA, Image Sensing Grp, Photon Div, CH-8048 Zurich, Switzerland

来源：

OPTICS EXPRESS | 2005年 / 13卷 / 24期

关键词：

D O I：

10.1364/OPEX.13.009812

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique that is increasingly being used in the life sciences during the past decades. However, a broader application of FLIM requires more cost-effective and user-friendly solutions. We demonstrate the use of a simple CCD/CMOS lock-in imager for fluorescence lifetime detection. The SwissRanger SR-2 time-of-flight detector, originally developed for 3D vision, embeds all the functionalities required for FLIM in a compact system. The further development of this technology and its combination with light-emitting-and laser diodes could drive a wider spreading of the use of FLIM including high-throughput applications. (c) 2005 Optical Society of America

引用

页码：9812 / 9821

页数：10

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