Rapid assay of A2058T-mutated 23S rRNA allelic profiles associated with high-level macrolide resistance in Moraxella catarrhalis

被引：2
|
作者：
Saito, Ryoichi [1 ]
Kasai, Ayako [1 ]
Ogihara, Shinji [2 ]
Yamada, Kageto [3 ]
Tao, Kazuyuki [4 ]
机构：
[1] Tokyo Med & Dent Univ, Grad Sch Hlth Care Sci, Dept Microbiol & Immunol, Tokyo, Japan
[2] Tokyo Med & Dent Univ, Hosp Med, Dept Clin Lab, Tokyo, Japan
[3] Tokyo Metropolitan Hlth & Med Corp Toshima Hosp, Dept Clin Lab, Tokyo, Japan
[4] Univ Tokyo, Radioisotope Ctr, Tokyo, Japan
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2015年 / 64卷
关键词：
MUTATIONS;
D O I：
10.1099/jmm.0.000130
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We report on a restriction fragment-length polymorphism (HpyCH4III) assay for profile analysis of 23S rRNA gene A2058T-mutated alleles associated with high-level macrolide resistance in Moraxella catarrhalis. Our assay results were supported by DNA sequencing analysis, allowed for simultaneous testing of many strains, and produced results from pure-cultured colonies within 4 h.
引用
收藏
页码：990 / 992
页数：3
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