In vitro activity of mastoparan-AF alone and in combination with clinically used antibiotics against multiple-antibiotic-resistant Escherichia coli isolates from animals
The in vitro activity of mastoparan-AF, an amphipathic antimicrobial peptide isolated from the hornet venom of Vespa affinis, alone and in combination with various clinically used antibiotics, was investigated against 21 Escherichia coli isolates/strains. Most E. coli isolates tested were detected containing multiple-antimicrobial resistance genes. Antimicrobial activity of mastoparan-AF was measured by MIC, MBC, time-kill kinetic assay and chequerboard titration method. Mastoparan-AF exhibited potent antimicrobial activity against most multiple-antibiotic-resistant E. coli isolates at the concentrations ranging from 4 to 16 mu g/ml. Combination studies showed that mastoparan-AF acts synergistically with certain antibiotics, i.e., cephalothin or gentamicin, against some multiple-antibiotic-resistant E. coli isolates. In conclusion, mastoparan-AF alone or in combination with other antibiotics could be promising as alternatives for combating multiple-antibiotic-resistant E. coli in future clinical applications. (C) 2012 Elsevier Inc. All rights reserved.
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Korea Univ, Ansan Hosp, Dept Med, Div Infect Dis,Coll Med, Ansan 15355, South KoreaKorea Univ, Ansan Hosp, Dept Med, Div Infect Dis,Coll Med, Ansan 15355, South Korea
Seok, Hyeri
Choi, Ji Young
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Sungkyunkwan Univ, Dept Microbiol, Sch Med, Suwon 16419, South KoreaKorea Univ, Ansan Hosp, Dept Med, Div Infect Dis,Coll Med, Ansan 15355, South Korea
Choi, Ji Young
Wi, Yu Mi
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Sungkyunkwan Univ, Samsung Changwon Hosp, Div Infect Dis, Sch Med, Chang Won 51353, South KoreaKorea Univ, Ansan Hosp, Dept Med, Div Infect Dis,Coll Med, Ansan 15355, South Korea
Wi, Yu Mi
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Park, Dae Won
Peck, Kyong Ran
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Sungkyunkwan Univ, Samsung Med Ctr, Div Infect Dis, Sch Med, Seoul 06351, South KoreaKorea Univ, Ansan Hosp, Dept Med, Div Infect Dis,Coll Med, Ansan 15355, South Korea
Peck, Kyong Ran
Ko, Kwan Soo
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Sungkyunkwan Univ, Dept Microbiol, Sch Med, Suwon 16419, South KoreaKorea Univ, Ansan Hosp, Dept Med, Div Infect Dis,Coll Med, Ansan 15355, South Korea