In vitro activity of mastoparan-AF alone and in combination with clinically used antibiotics against multiple-antibiotic-resistant Escherichia coli isolates from animals

被引：21
|
作者：
Lin, Chun-Hsien [1 ]
Hou, Roger F. [1 ]
Shyu, Ching-Lin [2 ,3 ]
Shia, Wei-Yau [2 ]
Lin, Chuen-Fu [4 ]
Tu, Wu-Chun [1 ]
机构：
[1] Natl Chung Hsing Univ, Dept Entomol, Taichung 40227, Taiwan
[2] Natl Chung Hsing Univ, Dept Vet Med, Taichung 40227, Taiwan
[3] Natl Chung Hsing Univ, Grad Inst Microbiol & Publ Hlth, Taichung 40227, Taiwan
[4] Cent Taiwan Univ Sci & Technol, Dept Med Lab Sci & Biotechnol, Taichung 40601, Taiwan
来源：
PEPTIDES | 2012年 / 36卷 / 01期
关键词：
Mastoparan-AF; Antimicrobial resistance gene; Antimicrobial activity; Antibiotic; E; coli; Synergism; ANTIMICROBIAL-RESISTANCE; BETA-LACTAMASES; KLEBSIELLA-PNEUMONIAE; GENE PROFILES; PEPTIDES; AGENTS; MECHANISMS; VENOM; CHLORAMPHENICOL; PREVALENCE;
D O I：
10.1016/j.peptides.2012.03.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The in vitro activity of mastoparan-AF, an amphipathic antimicrobial peptide isolated from the hornet venom of Vespa affinis, alone and in combination with various clinically used antibiotics, was investigated against 21 Escherichia coli isolates/strains. Most E. coli isolates tested were detected containing multiple-antimicrobial resistance genes. Antimicrobial activity of mastoparan-AF was measured by MIC, MBC, time-kill kinetic assay and chequerboard titration method. Mastoparan-AF exhibited potent antimicrobial activity against most multiple-antibiotic-resistant E. coli isolates at the concentrations ranging from 4 to 16 mu g/ml. Combination studies showed that mastoparan-AF acts synergistically with certain antibiotics, i.e., cephalothin or gentamicin, against some multiple-antibiotic-resistant E. coli isolates. In conclusion, mastoparan-AF alone or in combination with other antibiotics could be promising as alternatives for combating multiple-antibiotic-resistant E. coli in future clinical applications. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码：114 / 120
页数：7
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