Quality control assessment of human immunodeficiency virus type 2 (HIV-2) viral load quantification assays:: Results from an international collaboration on HIV-2 infection in 2006

被引:33
|
作者
Damond, Florence [1 ]
Benard, Antoine [2 ]
Ruelle, Jean [3 ]
Alabi, Abraham [4 ]
Kupfer, Bernd [5 ]
Gomes, Perpetua [7 ]
Rodes, Berta [6 ]
Albert, Jan [8 ,9 ]
Boeni, Juerg [10 ]
Garson, Jeremy [11 ]
Ferns, Bridget [11 ]
Matheron, Sophie [12 ]
Chene, Genevieve [2 ]
Vezinet, Francoise Brun [1 ]
机构
[1] Hop Bichat Claude Bernard, Virol Lab, APHP, F-75018 Paris, France
[2] Univ Bordeaux 2, INSERM, U897, ISPED, F-33076 Bordeaux, France
[3] Catholic Univ Louvain, AIDS Reference Lab, B-1348 Louvain, Belgium
[4] MRC Labs, Banjul, Gambia
[5] Inst Virol, Bonn, Germany
[6] Hosp Carlos III, Dept Infect Dis, Madrid, Spain
[7] CHLO Hosp Egas Moniz, Mol Biol Lab, Lisbon, Portugal
[8] Swedish Inst Infect Dis Control, Dept Virol, Solna, Sweden
[9] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden
[10] Swiss HIV Cohort Study, Zurich, Switzerland
[11] UCL Royal Free & Univ Coll Med Sch, Ctr Virol, London WC1E 6BT, England
[12] Hop Bichat Claude Bernard, Serv Malad Infect & Trop, APHP, F-75877 Paris 18, France
基金
英国医学研究理事会;
关键词
D O I
10.1128/JCM.00126-08
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human immunodeficiency virus type 2 (HIV-2) RNA quantification assays used in nine laboratories of the ACHIEV2E (A Collaboration on HIV-2 Infection) study group were evaluated. In a blinded experimental design, laboratories quantified three series of aliquots of an HIV-2 subtype A strain, each at a different theoretical viral load. Quantification varied between laboratories, and international standardization of quantification assays is strongly needed.
引用
收藏
页码:2088 / 2091
页数:4
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