Thioflavin T as luminescence biosensors for nucleic acid study and RNase A activity detection

被引：1

作者：

Du, Jinya ^{[1
]}

Dong, Yuzhi ^{[2
]}

Liu, Han ^{[1
]}

Gong, Lingli ^{[1
]}

Lu, Shuang ^{[1
]}

Yang, Changying ^{[1
]}

Li, Yang ^{[2
]}

机构：

[1] China Three Gorges Univ, Coll Biol & Pharmaceut Sci, Yichang 443002, Peoples R China

[2] Hubei Univ, Coll Life Sci, Wuhan 430062, Hubei, Peoples R China

来源：

MICROCHEMICAL JOURNAL | 2019年 / 147卷

基金：

中国国家自然科学基金;

关键词：

Thioflavin T (ThT); Fluorescence; RNA; RNase A; Inhibitor; DEOXYRIBONUCLEASE I ACTIVITY; LABEL-FREE; SENSITIVE DETECTION; GRAPHENE OXIDE; DNA-REPAIR; ASSAY; BINDING; DYE; MECHANISM; GROWTH;

D O I：

10.1016/j.microc.2019.04.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An applicable RNase assay was constructed based on strong luminescent complex between Thioflavin T (ThT) and RNA. The emission of ThT was more sensitive to RNA than to DNA, and electrostatic interaction played an important role when ThT interacted with RNA. The ThT-RNA fluorescence system displayed high specific quenching for RNase A, which originated from the specific cleavage reaction of RNA by RNase A. The quantitative detection of RNase A was built with a rather low detection limit 2.47 x 10(-6) U/mL. Enzymatic kinetics and inhibitory experiments were conducted to demonstrate the utilities of this method in RNase A activity study and screening of enzyme inhibitors in biological samples.

引用

页码：842 / 847

页数：6

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