Significant Expansion of the Fluorescent Protein Chromophore through the Genetic Incorporation of a Metal-Chelating Unnatural Amino Acid

被引：72

作者：

Liu, Xiaohong ^{[1
]}

Li, Jiasong ^{[1
,2
]}

Hu, Cheng ^{[1
]}

Zhou, Qing ^{[1
]}

Zhang, Wei ^{[1
]}

Hu, Meirong ^{[1
]}

Zhou, Juanzuo ^{[1
]}

Wang, Jiangyun ^{[1
]}

机构：

[1] Chinese Acad Sci, Lab Noncoding RNA, Inst Biophys, Beijing 100101, Peoples R China

[2] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 18期

基金：

美国国家科学基金会;

关键词：

amino acids; fluorescent proteins; genetic-code expansion; metal-chelating amino acids; metalloenzymes; CROSS-LINK; DYNAMICS; SENSORS; SITE;

D O I：

10.1002/anie.201301307

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Caught red-shifted: A novel metal-chelating unnatural amino acid with an 8-hydroxyquinoline group (HqAla) can be enzymatically incorporated into GFP (see scheme). Substituting a Tyr residue in the chromophore of FPs with HqAla results in significantly red-shifted excitation and emission maxima. The crystal structure of superfolder GFP bearing HqAla in its chromophore shows the structural basis for these red shifts. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：4805 / 4809

页数：5

共 50 条

[11] Genetic Incorporation of an Unnatural Fluorescent Amino Acid in a Plant H-ATPase Expressed in Yeast
Gayed, Bassem
Lee, Hyun Soo
Palmgren, Michael G.
Berlin, Joshua R.
BIOPHYSICAL JOURNAL, 2011, 100 (03) : 465 - 465
[12] RECOMBINANT PROTEIN STABILIZATION THROUGH ENGINEERED METAL-CHELATING SITES
UMANA, P
KELLIS, JT
ARNOLD, FH
ACS SYMPOSIUM SERIES, 1993, 516 : 102 - 108
[13] Exploring genetic codon expansion for unnatural amino acid incorporation in filamentous fungus Aspergillus nidulans
Li, Xueying
Wang, Jing
Li, Jingyi
Zhou, Yao
Huang, Xiaofei
Guo, Lingyan
Liu, Renning
Luo, Yiqing
Tan, Xinyu
Hu, Xiaotao
Gao, Yan
Yu, Bingzi
Fu, Mingxin
Wang, Ping
Zhou, Shengmin
JOURNAL OF BIOTECHNOLOGY, 2024, 393 : 91 - 99
[14] Imaging the proteome through the genetic incorporation of unnatural amino acids
Wang, Jiangyun
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 256
[15] Metalloprotein design through the genetic incorporation of unnatural amino acids
Wang, Jiangyun
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 246
[16] Unnatural amino acid mutagenesis of green fluorescent protein
Wang, L
Xie, JM
Deniz, AA
Schultz, PG
JOURNAL OF ORGANIC CHEMISTRY, 2003, 68 (01): : 174 - 176
[17] Site-specific incorporation of a fluorescent terphenyl unnatural amino acid
Lampkowski, Jessica S.
Uthappa, Diya M.
Young, Douglas D.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2015, 25 (22) : 5277 - 5280
[18] Genetic Incorporation of a Metal-Ion Chelating Amino Acid into Proteins as a Biophysical Probe
Lee, Hyun Soo
Spraggon, Glen
Schultz, Peter G.
Wang, Feng
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (07) : 2481 - +
[19] Genetic Incorporation of Fluorescent Amino Acid into Fatty Acid Binding Protein for Fatty Acid Detection
Yang, Ke
Yu, Minling
Zhu, Xiaolei
Xia, Yan
Li, Fahui
Li, Yongze
Liu, Xiaohong
Wang, Jiangyun
JOURNAL OF MOLECULAR BIOLOGY, 2022, 434 (08)
[20] Genetic incorporation of the photocrosslinking and redox active unnatural amino acid, anthraquinonylalanine
Hazen, Jennifer L.
Mehl, Ryan A.
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2006, 231

← 1 2 3 4 5 →