The Influence of Matrix Degradation and Functionality on Cell Survival and Morphogenesis in PEG-Based Hydrogels

被引：42

作者：

Raza, Asad ^{[1
]}

Lin, Chien-Chi ^{[1
]}

机构：

[1] Indiana Univ Purdue Univ, Dept Biomed Engn, Purdue Sch Engn & Technol, Indianapolis, IN 46202 USA

来源：

MACROMOLECULAR BIOSCIENCE | 2013年 / 13卷 / 08期

关键词：

cell; material interactions; degradation; hydrogels; mesenchymal stem cells; POLY(ETHYLENE GLYCOL) HYDROGELS; MESENCHYMAL STEM-CELLS; OSTEOGENIC DIFFERENTIATION; IN-VITRO; CHONDROGENIC DIFFERENTIATION; PEPTIDE; MODULUS; BIOMATERIALS; VIABILITY; CHEMISTRY;

D O I：

10.1002/mabi.201300044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two norbornene-functionalized PEG macromers are synthesized to render hydrogels with different hydrolytic degradability. Dithiol-containing linkers such as dithiothreitol or biscysteine-containing peptides are used to control proteolytic degradability. The influence of thiol-ene gel degradability on cell survival and morphogenesis in 3D is assessed using hMSCs and pancreatic MIN6 cells. The initial cell viability can be negatively affected in highly crosslinked thiol-ene hydrogels. When cells are encapsulated in thiol-ene gels lacking cell-adhesive motifs, their survival and proliferation are promoted in more hydrolytically labile hydrogels. The degree of 3D cell spreading in encapsulated hMSCs is enhanced when the matrices are immobilized with cell-adhesive motifs.

引用

页码：1048 / 1058

页数：11

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