Determination of Angiogenic Factors in Serum by Protein Array in Patients with Renal Cell Carcinoma

被引:2
|
作者
Lukesova, S. [2 ,6 ]
Kopecky, O. [1 ,2 ,6 ]
Vroblova, V. [3 ]
Hlavkova, D. [3 ]
Andrys, C. [3 ]
Moravek, P. [4 ]
Cermakova, E. [5 ]
机构
[1] Charles Univ Prague, Fac Med, Hradec Kralove 50005, Czech Republic
[2] Univ Teaching Hosp, Dept Internal Med 2, Hradec Kralove 50005, Czech Republic
[3] Univ Teaching Hosp, Inst Clin Immunol & Allergol, Hradec Kralove 50005, Czech Republic
[4] Univ Teaching Hosp, Dept Urol, Hradec Kralove 50005, Czech Republic
[5] Univ Teaching Hosp, Comp Technol Ctr, Hradec Kralove 50005, Czech Republic
[6] Dist Hosp Ndchod, Dept Oncol, Nachod, Czech Republic
关键词
renal cell carcinoma; angiogenin; PDGF; MCP-1; protein array method;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using the protein array method we determined the serum levels of a number of angiogenic factors. We identified serum levels of angiogenin, PDGF and MCP-1 (CCL2 chemokine) in serum of 32 patients with RCC, and 14 healthy volunteers by means of antibody array analysis. The patients were divided into three groups according to their disease stages (I+II, III, and IV). We found significant differences between the controls and patients with RCC both pre-operatively and post-operatively in angiogenin, PDGF and MCP-1 serum levels. The increase in angiogenin, PDGF and MCP-1 lasted in patients with RCC stages I-III even without metastases eight weeks post-operatively. The patients with stage IV RCC showed disturbed production of PDGF and MCP-1. Protein array analysis is a powerful tool for the identification of large numbers of trace proteins. Multiplex antibody array is able to provide data more precisely reflecting the nature of pathological processes.
引用
收藏
页码:134 / 140
页数:7
相关论文
共 50 条
  • [21] Serum immunosuppressive acidic protein as a potent prognostic factor for patients with metastatic renal cell carcinoma
    Igarashi, T
    Tobe, T
    Kuramochi, H
    Akakura, K
    Ichikawa, T
    Hamano, S
    Suzuki, N
    Furuya, Y
    Ito, H
    JAPANESE JOURNAL OF CLINICAL ONCOLOGY, 2001, 31 (01) : 13 - 17
  • [22] SERUM ANTIBODY AGAINST TAMM-HORSFALL PROTEIN IN PATIENTS WITH RENAL-CELL CARCINOMA
    FOWLER, JE
    FRIERSON, HF
    MILLS, SE
    MARIANO, M
    CANCER, 1987, 59 (11) : 1923 - 1926
  • [23] Prognostic Factors in Patients With Advanced Renal Cell Carcinoma
    Muriel Lopez, Carolina
    Esteban, Emilio
    Pablo Berros, Jose
    Pardo, Pablo
    Astudillo, Aurora
    Izquierdo, Marta
    Crespo, Guillermo
    Sanmamed, Miguel
    Fonseca, Paula J.
    Martinez-Camblor, Pablo
    CLINICAL GENITOURINARY CANCER, 2012, 10 (04) : 262 - 270
  • [24] Prognostic Factors in Patients With Advanced Renal Cell Carcinoma
    Muriel, C.
    Esteban, E.
    Astudillo, A.
    Martinez-Camblor, P.
    Corral, N.
    Crespo, G.
    Berros, J. P.
    Fonseca, P. J.
    Luque, M.
    Lacave, A. J.
    EUROPEAN JOURNAL OF CANCER, 2011, 47 : S523 - S523
  • [25] Differential expression and clinical significance of serum protein among patients with clear-cell renal cell carcinoma
    Nuerrula, Yiliyaer
    Rexiati, Mulati
    Liu, Qiang
    Wang, Yu-Jie
    CANCER BIOMARKERS, 2015, 15 (04) : 485 - 491
  • [26] Cytokines and angiogenic factors in metastatic renal cell cancer: Association of pretreatment serum levels with survival
    Montero, A. J.
    Diaz-Montero, C. M.
    Liu, J.
    Do, K.
    Millikan, R. E.
    Tannir, N. M.
    JOURNAL OF CLINICAL ONCOLOGY, 2009, 27 (15)
  • [27] THE PROGNOSIC IMPORTANCE OF ANGIOGENIC PHENOTYPE IN RENAL CELL CARCINOMA
    Bigot, Pierre
    Rioux-Leclercq, Nathalie
    Bensalah, Karim
    Edeline, Julien
    Vigneau, Cecile
    Martin, Benedicte
    Jouan, Florence
    Denis, Marc
    Patard, Jean-Jacques
    JOURNAL OF UROLOGY, 2009, 181 (04): : 111 - 111
  • [28] Anti-Angiogenic Therapy in Renal Cell Carcinoma
    Sharma, Shree G.
    Nanda, Sudip
    Longo, Santo
    RECENT PATENTS ON ANTI-CANCER DRUG DISCOVERY, 2010, 5 (01) : 77 - 83
  • [29] THE PROGNOSIC IMPORTANCE OF ANGIOGENIC PHENOTYPE IN RENAL CELL CARCINOMA
    Bigot, P.
    Edeline, J.
    Vigneau, C.
    Martin, B.
    Jouan, F.
    Denis, M.
    Patard, J. J.
    Rioux-Leclercq, N.
    EUROPEAN UROLOGY SUPPLEMENTS, 2009, 8 (04) : 298 - 298
  • [30] Role of molecular factors in renal cell carcinoma: a tissue array-analysis
    Anton Aparicio, L. M.
    Medina Villaamil, V.
    Valbuena Ruvira, L.
    Rey Sanjurjo, J. L.
    Diaz Prado, S. M.
    Valladares Ayerbes, M.
    Aparicio Gallego, G.
    Santamarina Cainzos, I.
    Haz Conde, M.
    Grande Pulido, E.
    JOURNAL OF CLINICAL ONCOLOGY, 2008, 26 (15)