RNAi as a tool for understanding germline development in Caenorhabditis elegans:: Uses and cautions

被引：18
|
作者：
Maine, EM [1 ]
机构：
[1] Syracuse Univ, Dept Biol, Syracuse, NY 13244 USA
来源：
DEVELOPMENTAL BIOLOGY | 2001年 / 239卷 / 02期
关键词：
Caenorhabditis elegans; C; elegans; RNA interference; RNAi; germ line; germ cells; development; germline development;
D O I：
10.1006/dbio.2001.0394
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
RNA-mediated genetic interference (RNAi) has become a very useful tool for analyzing gene function in development and other processes. RNAi can be used as a complement to traditional genetic studies or as a primary means of determining biological function. However, the efficacy of RNAi depends on a variety of factors that the researcher must take into consideration. This review focuses on germline development in the nematode, Caenorhabditis elegans, and discusses the uses and limitations of RNAi in providing new information about gene function as well as the possible endogenous role RNAi plays in germline physiology. (C) 2001 Academic Press.
引用
收藏
页码：177 / 189
页数：13
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