Isolation of a novel stable peptide from cultivated Raphanus sativus with peroxidase activity

被引：2

作者：

Omumi, A

Morshedi, D

Samadi, A

机构：

[1] Pasteur Inst Iran, Dept Biochem, Tehran, Iran

[2] Natl Res Ctr Genet Engn & Biotechnol, Tehran, Iran

[3] Kirksville Coll Osteopath Med, Dept Biochem, Kirksville, MO 63501 USA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2001年 / 17卷 / 08期

关键词：

aqueous two phase partitioning; peroxidase; Raphanus sativus;

D O I：

10.1023/A:1013563107869

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Isolation of a novel stable peptide from cultivated Raphanus sativus with peroxidase activity has been achieved using zinc ion precipitation followed by aqueous two phase extraction with a polyethylene glycol (PEG)/phosphate system. The best result was achieved with PEG 600/phosphate (28.8/13% w/v) which extracted 96.15% of total enzyme activity. The peptide has a molecular weight of 5.85 kD as determined by HPLC size exclusion method with an optimized pH of 6.

引用

页码：827 / 828

页数：2

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