Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation

被引：25
|
作者：
Jaber-Hijazi, Farah [1 ]
Lo, Priscilla J. K. P. [2 ]
Mihaylova, Yuliana [1 ]
Foster, Jeremy M. [3 ]
Benner, Jack S. [3 ]
Romero, Belen Tejada [1 ]
Chen, Chen [2 ]
Malla, Sunir [2 ]
Solana, Jordi [2 ]
Ruzov, Alexey [2 ]
Aboobaker, A. Aziz [1 ]
机构：
[1] Univ Oxford, Dept Zool, Oxford OX1 3PS, England
[2] Univ Nottingham, Queens Med Ctr, Nottingham NG7 2UH, England
[3] New England Biolabs Inc, Ipswich, MA 01938 USA
来源：
DEVELOPMENTAL BIOLOGY | 2013年 / 384卷 / 01期
基金：
英国生物技术与生命科学研究理事会;
关键词：
Methyl binding domain; Pluripotency; Differentiation; Regeneration; CPG-BINDING DOMAIN; SCHMIDTEA-MEDITERRANEA; GENE-EXPRESSION; CAENORHABDITIS-ELEGANS; PRISTIONCHUS-PACIFICUS; MAMMALIAN DEVELOPMENT; NERVOUS-SYSTEM; COMPLEX; REGENERATION; NURD;
D O I：
10.1016/j.ydbio.2013.09.020
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Planarian adult stem cells (pASCs) or neoblasts represent an ideal system to study the evolution of stem cells and pluripotency as they underpin an unrivaled capacity for regeneration. We wish to understand the control of differentiation and pluripotency in pASCs and to understand how conserved, convergent or divergent these mechanisms are across the Bilateria. Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis. The genome does not have detectable levels of 5-methylcytosine (5(m)C) and we find no role for a potential DNA methylase. We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码：141 / 153
页数：13
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