In-cell covalent labeling of reactive His-tag fused proteins

被引：40

作者：

Uchinomiya, Shohei ^{[1
]}

Nonaka, Hiroshi ^{[2
]}

Wakayama, Sho ^{[1
]}

Ojida, Akio ^{[3
]}

Hamachi, Itaru ^{[1
,4
]}

机构：

[1] Kyoto Univ, Dept Synthet Chem & Biol Chem, Katsura, Kyoto 6158510, Japan

[2] Kyushu Univ, INAMORI Frontier Res Ctr, Nishi Ku, Fukuoka 8190395, Japan

[3] Kyushu Univ, Grad Sch Pharmaceut Sci, Higashi Ku, Fukuoka 8128582, Japan

[4] Japan Sci & Technol Agcy JST, CREST, Chiyoda Ku, Tokyo 1020075, Japan

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 44期

关键词：

RECOGNITION; MOLECULES; CHEMISTRY; SURFACE; PROBE;

D O I：

10.1039/c3cc41979g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new method for in-cell protein labeling was developed. This method employed a binding-induced nucleophilic reaction between the Cys-appended His-tag and the Ni(II)-NTA containing an a-chloroacetamide. Using this method, not only labeling of His-tag fused proteins but also the detection of a protein-protein interaction was achieved inside living cells.

引用

页码：5022 / 5024

页数：3

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