ISOLATION AND CHARACTERIZATION OF POLYMORPHIC MICROSATELLITES IN IRIS ENSATA (IRIDACEAE)

被引：2
|
作者：
Xiao, Yue-E [1 ,2 ]
Hu, Yong-Hong [2 ]
Liu, Min [1 ]
Chen, Xiao-Yong [1 ]
机构：
[1] E China Normal Univ, Sch Resources & Environm Sci, Shanghai 200062, Peoples R China
[2] Chinese Acad Sci, Shanghai Chenshan Plant Sci Res Ctr, Chenshan Bot Garden, Shanghai 201602, Peoples R China
来源：
AMERICAN JOURNAL OF BOTANY | 2012年 / 99卷 / 12期
关键词：
Iris ensata; microsatellite markers; perennial herb; polymorphism; PRIMERS;
D O I：
10.3732/ajb.1200266
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Premise of the study: Microsatellite primers were developed in Iris ensata (Iridaceae) to provide polymorphic markers for further studies into population genetics. Methods and Results: Thirteen polymorphic microsatellite loci were isolated from I. ensata. These loci were successfully amplified in two natural populations of I. ensata from eastern China (Longwangshan, Zhejiang Province) and northeastern China (Jinchuan, Jilin Province). There was no significant linkage disequilibrium found for any pair of loci. These loci contained between two and 12 alleles per locus across all 48 individuals of I. ensata. The number of alleles per locus varied from two to 10 at the population level and the observed and expected heterozygosities ranged from 0.167 to 0.958 and from 0.284 to 0.853, respectively. Conclusions: These loci showed high levels of polymorphism and could be used to study the population genetic structure, genetic relationships, and phylogeography of I. ensata.
引用
收藏
页码：E498 / E500
页数：3
相关论文
共 50 条
  • [31] Isolation and characterization of fifteen polymorphic tetranucleotide microsatellites for Ichthyophis bannanicus (Gymnophiona: Ichthyophiidae)
    Guifen Li
    Xia Luo
    Hui Wang
    Yongjian Bei
    Wenbo Shi
    Shaoquan Meng
    Baowei Zhang
    Conservation Genetics Resources, 2010, 2 : 129 - 131
  • [32] Isolation and characterization of 17 polymorphic microsatellites in the brown mud mussel Glauconome rugosa
    Sun, Fei
    Zhang, Quanqi
    Tan, Juraini
    Lin, Grace
    Yue, Gen Hua
    CONSERVATION GENETICS RESOURCES, 2013, 5 (01) : 1 - 3
  • [33] Isolation and characterization of eight polymorphic microsatellites for the spotted spiny lobster, Panulirus guttatus
    Truelove, Nathan
    Behringer, Donald C.
    Butler, Mark J.
    Preziosi, Richard F.
    PEERJ, 2016, 4
  • [34] Isolation and characterization of polymorphic microsatellites for the natural populations of barker frog Physalaemus cuvieri
    Conte, M.
    Cancado, L. J.
    Laborda, P. R.
    Zucchi, M. I.
    Andrade, G. V.
    Rossa-Feres, D. C.
    Siqueira, S.
    Souza, A. P.
    Recco-Pimentel, S. M.
    CONSERVATION GENETICS, 2009, 10 (06) : 1849 - 1852
  • [35] Isolation and characterization of highly polymorphic microsatellites in the aquatic plant, Nymphoides peltata (Menyanthaceae)
    Uesugi, R
    Tani, N
    Goka, K
    Nishihiro, J
    Tsumura, Y
    Washitani, I
    MOLECULAR ECOLOGY NOTES, 2005, 5 (02): : 343 - 345
  • [36] Isolation and characterization of polymorphic microsatellites in the specialist grasshopper Ramburiella hispanica (Orthoptera: Acrididae)
    Maria Pilar Aguirre
    Víctor Noguerales
    Pedro J. Cordero
    Joaquín Ortego
    Conservation Genetics Resources, 2014, 6 : 723 - 724
  • [37] Isolation and characterization of polymorphic microsatellites for the natural populations of barker frog Physalaemus cuvieri
    M. Conte
    L. J. Cançado
    P. R. Laborda
    M. I. Zucchi
    G. V. Andrade
    D. C. Rossa-Feres
    S. Siqueira
    A. P. Souza
    S. M. Recco-Pimentel
    Conservation Genetics, 2009, 10 : 1849 - 1852
  • [38] Isolation and characterization of 17 polymorphic microsatellites in the brown mud mussel Glauconome rugosa
    Fei Sun
    Quanqi Zhang
    Juraini Tan
    Grace Lin
    Gen Hua Yue
    Conservation Genetics Resources, 2013, 5 : 1 - 3
  • [39] Isolation and characterization of polymorphic microsatellites from red coral grouper (Plectropomus maculatus)
    Zhu, ZY
    Lo, LC
    Lin, G
    Xu, YX
    Yue, GH
    MOLECULAR ECOLOGY NOTES, 2005, 5 (03): : 579 - 581
  • [40] Isolation and characterization of polymorphic trinucleotide microsatellites of the polyploid crucian carp (Carassius auratus)
    Jung, Jongwoo
    Lee, Eunjung
    Kim, Won
    MOLECULAR ECOLOGY NOTES, 2007, 7 (01): : 124 - 126
12345