Blautia stercoris sp nov., isolated from human faeces

Strain GAM6-1(T) is a novel, strictly anaerobic, non-spore-forming, Gram-stain-positive bacterium that was isolated from the faeces of a healthy individual. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GAM6-1(T) was most closely related to Blautia producta ATCC 27340(T) (95.7% sequence similarity), in the family Lachnospiraceae. Strain GAM6-1T did not exhibit catalase or oxidase activity. The strain's cellular fatty acids were of the straight-chain saturated and mono-unsaturated types, with C-14:0 (24.10%), C-16:0 (19.09%) and C-16:0 dimethylacetal (14.35%) predominant. Strain GAM6-1(T) was able to produce acid from various carbohydrates. Glucose fermentation produced acetic acid as the major short-chain fatty acid. The genomic DNA G + C content of strain GAM6-1(T) was 35.6 mol%. Based on phenotypic, genotypic and phylogenetic evidence, strain GAM6-1(T) (=KCTC 5981(T) =JCM 17204(T)) is considered to represent a novel species, for which the name Blautia stercoris sp. nov. is proposed.