Enhanced top-down characterization of histone post-translational modifications

被引：2

作者：

Tian, Zhixin ^{[1
,2
]}

Tolic, Nikola ^{[1
]}

Zhao, Rui ^{[1
]}

Moore, Ronald J. ^{[3
]}

Hengel, Shawna M. ^{[1
]}

Robinson, Errol W. ^{[1
]}

Stenoien, David L. ^{[3
]}

Wu, Si ^{[1
]}

Smith, Richard D. ^{[3
]}

Pasa-Tolic, Ljiljana ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA

[2] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China

[3] Pacific NW Natl Lab, Biol Sci Div, Richland, WA 99352 USA

来源：

GENOME BIOLOGY | 2012年 / 13卷 / 10期

关键词：

Saltless WCX-HILIC; top-down; histone; posttranslational modification; MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; H4; ISOFORMS; REPAIR; DOMAIN; CODE; H3;

D O I：

10.1186/gb-2012-13-10-R86

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) platform for high-throughput and sensitive characterization of histone PTMs at the intact protein level. The platform enables unambiguous identification of 708 histone isoforms from a single 2D LC-MS/MS analysis of 7.5 mu g purified core histones. The throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms.

引用

页数：9

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