Enhanced top-down characterization of histone post-translational modifications

被引：2
|
作者：
Tian, Zhixin [1 ,2 ]
Tolic, Nikola [1 ]
Zhao, Rui [1 ]
Moore, Ronald J. [3 ]
Hengel, Shawna M. [1 ]
Robinson, Errol W. [1 ]
Stenoien, David L. [3 ]
Wu, Si [1 ]
Smith, Richard D. [3 ]
Pasa-Tolic, Ljiljana [1 ]
机构：
[1] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA
[2] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
[3] Pacific NW Natl Lab, Biol Sci Div, Richland, WA 99352 USA
来源：
GENOME BIOLOGY | 2012年 / 13卷 / 10期
关键词：
Saltless WCX-HILIC; top-down; histone; posttranslational modification; MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; H4; ISOFORMS; REPAIR; DOMAIN; CODE; H3;
D O I：
10.1186/gb-2012-13-10-R86
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Post-translational modifications (PTMs) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. We report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) platform for high-throughput and sensitive characterization of histone PTMs at the intact protein level. The platform enables unambiguous identification of 708 histone isoforms from a single 2D LC-MS/MS analysis of 7.5 mu g purified core histones. The throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms.
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页数：9
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