Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)

被引：3

作者：

Lai, Meng Yee ^{[1
]}

Bukhari, Fatma Diyana Mohd ^{[1
]}

Zulkefli, Nur Zulaikha ^{[1
]}

Ismail, Ilyiana ^{[2
]}

Mustapa, Nur Izati ^{[2
]}

Soh, Tuan Suhaila Tuan ^{[2
]}

Hassan, Afifah Haji ^{[2
]}

Peariasamy, Kalaiarasu M. ^{[3
]}

Lee, Yee Leng ^{[4
]}

Suppiah, Jeyanthi ^{[5
]}

Thayan, Ravindran ^{[5
]}

Isa, Mohd Khairi Mat ^{[6
]}

Wahid, Nur Zafirah Abdul ^{[6
]}

Lau, Yee Ling ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Parasitol, Kuala Lumpur 50603, Malaysia

[2] Minist Hlth, Hosp Sungai Buloh, Dept Pathol, Kuala Lumpur, Malaysia

[3] Minist Hlth, Inst Clin Res, Natl Inst Hlth, Kuala Lumpur, Malaysia

[4] Minist Hlth, Clin Res Ctr, Hosp Sungai Buloh, Kuala Lumpur, Malaysia

[5] Minist Hlth, Infect Dis Res Ctr, Natl Inst Hlth, Virol Unit,Inst Med Res, Kuala Lumpur, Malaysia

[6] Selia Tek Holdings Sdn Bhd, Lot 18,Jalan Teknol 3-5, Kota Damansara 47810, Selangor, Malaysia

来源：

BMC INFECTIOUS DISEASES | 2022年 / 22卷 / 01期

关键词：

Diagnosis; RT-LAMP; SARS-CoV-2; Phenol red; pH sensitive indicator; VISUAL DETECTION;

D O I：

10.1186/s12879-022-07684-w

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples. Methods A pH sensitive dye was used as the end point detection method. The obvious colour changes between positive and negative reactions eliminates the need of other equipment. Results Clinical testing using 260 samples showed 92.7% sensitivity (95% CI 87.3-96.3%) and 93.6% specificity (95% CI 87.3-97.4%) of RT-LAMP. Conclusions The simple RNA extraction method minimizes the need for any extensive laboratory set-up. We suggest combining this simple nucleic acid extraction method and RT-LAMP technology as the point-of care diagnostic tool.

引用

页数：5

共 50 条

[1] Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)
Meng Yee Lai
Fatma Diyana Mohd Bukhari
Nur Zulaikha Zulkefli
Ilyiana Ismail
Nur Izati Mustapa
Tuan Suhaila Tuan Soh
Afifah Haji Hassan
Kalaiarasu M. Peariasamy
Yee Leng Lee
Jeyanthi Suppiah
Ravindran Thayan
Mohd Khairi Mat Isa
Nur Zafirah Abdul Wahid
Yee Ling Lau
[J]. BMC Infectious Diseases, 22
[2] Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP) (vol 22, 697, 2022)
Lau, Yee Ling
[J]. BMC INFECTIOUS DISEASES, 2023, 23 (01)
[3] Correction to: Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP) & Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
Yee Ling Lau
[J]. BMC Infectious Diseases, 23 (1)
[4] Colorimetric and fluorometric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for diagnosis of SARS-CoV-2
Galyah Alhamid
Huseyin Tombuloglu
Dalal Motabagani
Dana Motabagani
Ali A. Rabaan
Kubra Unver
Gabriel Dorado
Ebtesam Al-Suhaimi
Turgay Unver
[J]. Functional & Integrative Genomics, 2022, 22 : 1391 - 1401
[5] Colorimetric and fluorometric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for diagnosis of SARS-CoV-2
Alhamid, Galyah
Tombuloglu, Huseyin
Motabagani, Dalal
Motabagani, Dana
Rabaan, Ali A.
Unver, Kubra
Dorado, Gabriel
Al-Suhaimi, Ebtesam
Unver, Turgay
[J]. FUNCTIONAL & INTEGRATIVE GENOMICS, 2022, 22 (06) : 1391 - 1401
[6] RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Lai, Meng Yee
Suppiah, Jeyanthi
Thayan, Ravindran
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Lau, Yee Ling
[J]. TROPICAL MEDICINE AND HEALTH, 2022, 50 (01)
[7] Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Lena Mautner
Christin-Kirsty Baillie
Heike Marie Herold
Wolfram Volkwein
Patrick Guertler
Ute Eberle
Nikolaus Ackermann
Andreas Sing
Melanie Pavlovic
Ottmar Goerlich
Ulrich Busch
Lars Wassill
Ingrid Huber
Armin Baiker
[J]. Virology Journal, 17
[8] Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Mautner, Lena
Baillie, Christin-Kirsty
Herold, Heike Marie
Volkwein, Wolfram
Guertler, Patrick
Eberle, Ute
Ackermann, Nikolaus
Sing, Andreas
Pavlovic, Melanie
Goerlich, Ottmar
Busch, Ulrich
Wassill, Lars
Huber, Ingrid
Baiker, Armin
[J]. VIROLOGY JOURNAL, 2020, 17 (01)
[9] RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Meng Yee Lai
Jeyanthi Suppiah
Ravindran Thayan
Ilyiana Ismail
Nur Izati Mustapa
Tuan Suhaila Tuan Soh
Afifah Haji Hassan
Kalaiarasu M. Peariasamy
Yee Leng Lee
Yee Ling Lau
[J]. Tropical Medicine and Health, 50
[10] Rapid Detection of Tembusu Virus by Reverse-Transcription, Loop-mediated Isothermal Amplification (RT-LAMP)
Tang, Y.
Diao, Y.
Yu, C.
Gao, X.
Chen, L.
Zhang, D.
[J]. TRANSBOUNDARY AND EMERGING DISEASES, 2012, 59 (03) : 208 - 213

← 1 2 3 4 5 →