Composition of Mineral Produced by Dental Mesenchymal Stem Cells

被引:37
|
作者
Volponi, A. A. [1 ]
Gentleman, E. [1 ]
Fatscher, R. [2 ]
Pang, Y. W. Y. [1 ]
Gentleman, M. M. [2 ]
Sharpe, P. T. [1 ]
机构
[1] Kings Coll London, Inst Dent, Craniofacial Dev & Stem Cell Biol, London, England
[2] SUNY Stony Brook, Dept Mat Sci & Engn, Stony Brook, NY 11794 USA
基金
英国惠康基金;
关键词
MSCs; dental stem cells; dentine; cementum; Raman spectroscopy; mineralization; EXFOLIATED DECIDUOUS TEETH; HUMAN GINGIVAL FIBROBLASTS; PERIODONTAL-LIGAMENT; RAMAN-SPECTROSCOPY; TISSUE FORMATION; STROMAL CELLS; ENAMEL MATRIX; CORTICAL BONE; PULP CELLS; IN-VITRO;
D O I
10.1177/0022034515599765
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Mesenchymal stem cells isolated from different dental tissues have been described to have osteogenic/odontogenic-like differentiation capacity, but little attention has been paid to the biochemical composition of the material that each produces. Here, we used Raman spectroscopy to analyze the mineralized materials produced in vitro by different dental cell populations, and we compared them with the biochemical composition of native dental tissues. We show that different dental stem cell populations produce materials that differ in their mineral and matrix composition and that these differ from those of native dental tissues. In vitro, BCMP (bone chip mass population), SCAP (stem cells from apical papilla), and SHED (stem cells from human-exfoliated deciduous teeth) cells produce a more highly mineralized matrix when compared with that produced by PDL (periodontal ligament), DPA (dental pulp adult), and GF (gingival fibroblast) cells. Principal component analyses of Raman spectra further demonstrated that the crystallinity and carbonate substitution environments in the material produced by each cell type varied, with DPA cells, for example, producing a more carbonate-substituted mineral and with SCAP, SHED, and GF cells creating a less crystalline material when compared with other dental stem cells and native tissues. These variations in mineral composition reveal intrinsic differences in the various cell populations, which may in turn affect their specific clinical applications.
引用
收藏
页码:1568 / 1574
页数:7
相关论文
共 50 条
  • [41] Inflammatory Cytokines and Biodegradable Scaffolds in Dental Mesenchymal Stem Cells Priming
    Kanjevac, Tatjana
    Gustafson, Collin
    Ivanovska, Ana
    Ravanetti, Francesca
    Cacchioli, Antonio
    Bosnakovski, Darko
    CURRENT STEM CELL RESEARCH & THERAPY, 2019, 14 (04) : 320 - 326
  • [42] DENTAL MESENCHYMAL STEM CELLS MAY HAVE IMMUNOREGULATORY EFFECT ON ALLOREJECTION
    Gul, Fethi
    Dogan, Deniz
    Kasapoglu, Umut Sabri
    Akkoc, Tunc
    Cinel, Ismail
    TRANSPLANT INTERNATIONAL, 2017, 30 : 394 - 394
  • [43] Immunomodulatory properties of dental tissue-derived mesenchymal stem cells
    Li, Z.
    Jiang, C-M
    An, S.
    Cheng, Q.
    Huang, Y-F
    Wang, Y-T
    Gou, Y-C
    Xiao, L.
    Yu, W-J
    Wang, J.
    ORAL DISEASES, 2014, 20 (01) : 25 - 34
  • [44] Treatment of critical defects produced in calvaria of mice with mesenchymal stem cells
    Monteiro, Betania S.
    Argolo-Neto, Napoleao M.
    Nardi, Nance B.
    Chagastelles, Pedro C.
    Carvalho, Pablo H.
    Bonfa, Laila P.
    Flgueiras, Richard R.
    Reis, Amanda S.
    Del Carlo, Ricardo J.
    ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, 2012, 84 (03): : 841 - 851
  • [45] CGMP produced mesenchymal stem cells (allogen) support in vitro hematopoiesis
    Sumner, M
    Guevarra, C
    Simonetti, D
    Jennings, M
    Deans, R
    MacKay, A
    BONE MARROW TRANSPLANTATION, 2001, 27 : S322 - S322
  • [46] Mesenchymal Stem Cells and Endothelial Progenitor Cells Stimulate Bone Regeneration and Mineral Density
    Zigdon-Giladi, Hadar
    Bick, Tova
    Lewinson, Dina
    Machtei, Eli E.
    JOURNAL OF PERIODONTOLOGY, 2014, 85 (07) : 984 - 990
  • [47] Human dental follicle cells express embryonic, mesenchymal and neural stem cells markers
    Lima, Rodrigo Lopes
    Holanda-Afonso, Rosenilde Carvalho
    Moura-Neto, Vivaldo
    Bolognese, Ana Maria
    DosSantos, Marcos Fabio
    Souza, Margareth Maria
    ARCHIVES OF ORAL BIOLOGY, 2017, 73 : 121 - 128
  • [48] GENERATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS FROM DENTAL PULP-DERIVED MESENCHYMAL STEM CELLS
    Lee, J. H.
    Lee, Y. M.
    Maeng, G. H.
    Jeon, R. H.
    Kim, T. H.
    Lee, W. J.
    Ock, S. A.
    Jeon, B. G.
    Kumar, B. Mohana
    Lee, S. L.
    Rho, G. J.
    REPRODUCTION FERTILITY AND DEVELOPMENT, 2012, 24 (01) : 215 - 215
  • [49] Mitochondrial genome mutations in mesenchymal stem cells derived from human dental induced pluripotent stem cells
    Park, Jumi
    Lee, Yeonmi
    Shin, Joosung
    Lee, Hyeon-Jeong
    Son, Young-Bum
    Park, Bong-Wook
    Kim, Deokhoon
    Rho, Gyu-Jin
    Kang, Eunju
    BMB REPORTS, 2019, 52 (12) : 689 - 694
  • [50] Molecular composition of cell-cell contact between hematopoietic stem cells and mesenchymal stem cells
    Wagner, W.
    Wein, F.
    Roderburg, C.
    Benes, V.
    Frankhauser, M.
    Wuchter, P.
    Eckstein, V.
    Ho, A. D.
    BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, 2007, 13 (02) : 47 - 48