The use of Box-Behnken design of experiments to study in vitro salt tolerance by Pisolithus tinctorius

被引：24

作者：

Nagarajan, G ^{[1
]}

Natarajan, K ^{[1
]}

机构：

[1] Univ Madras, Ctr Adv Study Bot, Madras 600025, Tamil Nadu, India

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1999年 / 15卷 / 02期

关键词：

Box-Behnken design; salt tolerance; Pisolithus tinctorius;

D O I：

10.1023/A:1008899204502

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ectomycorrhizal fungus Pisolithus tinctorius was grown in vitro with different concentrations and combinations of three different sodium salts viz., sodium chloride, sodium sulphate and trisodium citrate (NaCl, Na2SO4 and Na3C6H5O7) for 30 days. At the end of the experiment the mycelial biomass and protein content of the fungus was evaluated. Based on the salts tested the combination of NaCl and Na2SO4 in optimum concentrations actually promoted the growth of P. tinctorius. Box-Behnken design with three variables like NaCl, Na2SO4 and Na3C6H5O7 was used to identify a significant correlation between the effect of these variables on mycelial biomass production. The experimental values are in good agreement with predicted values, the correlation coefficient being 0.9880.

引用

页码：197 / 203

页数：7

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