Investigation of cell adhesion to structured surfaces using total internal reflection fluorescence and confocal laser scanning microscopy

被引：5
|
作者：
Joos, U
Biskup, T
Ernst, O
Westphal, I
Gherasim, C
Schmidt, R
Edinger, K
Pilarczyk, G
Duschl, C
机构：
[1] Fraunhofer Inst Biomed Engn, D-10115 Berlin, Germany
[2] NaWoTec GmbH, D-64380 Rossdorf, Germany
来源：
EUROPEAN JOURNAL OF CELL BIOLOGY | 2006年 / 85卷 / 3-4期
关键词：
focal adhesion; surface patterning; TIRF microscopy; focal adhesion kinase;
D O I：
10.1016/j.ejcb.2005.08.007
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Adhesion of adherent cells oil structured surfaces is influenced by the surface pattern given. Here, we designed a structured gold relief surface based on cell adhesion patterns we had previously observed. We analysed the geometric parameters and the overall distribution of focal adhesion kinase in focal adhesions on unstructured glass surfaces using optical microscopy. The basic structural elements obtained from this analysis were arranged in regular Clusters that resembled the shape of a polarised migratory cell. In time-lapse studies we observed that the cells adhere preferentially to the gold pads and adopt the shape of the clusters. Staining of the actin cytoskeleton revealed that the actin filaments are aligned to the gold pads of the elementary structure. (c) 2005 Elsevier GmbH. All rights reserved.
引用
收藏
页码：225 / 228
页数：4
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