Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry

被引：41

作者：

Ishizuka, Takumi ^{[1
]}

Kimoto, Michiko ^{[1
,2
]}

Sato, Akira ^{[1
]}

Hirao, Ichiro ^{[1
,2
]}

机构：

[1] RIKEN, SSBC, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[2] TagCyx Biotechnol, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 88期

基金：

日本学术振兴会;

关键词：

POSTTRANSCRIPTIONAL CHEMICAL FUNCTIONALIZATION; ENZYMATIC INCORPORATION; GENETIC ALPHABET; TERMINAL ALKYNES; MODIFIED DNA; IN-VIVO; DYNAMICS; RIBOSOME; CYCLOADDITION; TRANSLATION;

D O I：

10.1039/c2cc36293g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Toward new biotechnology by genetic alphabet expansion, we developed an efficient site-specific labeling method for large RNA molecules. The combination of unnatural base pair transcription and post-transcriptional modification by click chemistry enables simple RNA labeling with a wide variety of functional groups at desired positions, in a one-pot reaction.

引用

页码：10835 / 10837

页数：3

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