Transgenic tobacco plants harboring tomato proteinase inhibitor II gene and their insect resistance

被引：0
|
作者：
Xie, XZ
Huang, MJ
Wu, NH [1 ]
机构：
[1] Chinese Acad Sci, Inst Dev Biol, Beijing 100080, Peoples R China
[2] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
来源：
PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL | 2002年 / 12卷 / 03期
关键词：
tomato proteinase inhibitor II gene; transgenic tobacco; insect-resistance;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The plant expression vectors pBCT2 and pBT2 were constructed with the cDNA sequence ( tin2) and genomic DNA sequence (tin2i) of tomato proteinase inhibitor II gene respectively. Then the two expression vectors were transferred into tobacco via the Agrobacterium tumefaciens strain LBA4404, and transgenic tobacco plants were generated. Molecular analysis and trypsin activity assay showed that both cDNA and genomic DNA were expressed properly in the transgenic plants. Insecticidal activities in these transgenic plants indicated that transgenic tobacco plants carrying tin2i sequence were more resistant to 2-instar larvae of Heliothis armigera Hubner than those carrying tin2 sequence. Therefore the intron of tin2i sequence might be a contributor to insecticidal activity of the transgenic tobacco.
引用
收藏
页码：198 / 203
页数：6
相关论文
共 50 条
  • [41] The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic tobacco plants
    Rafael Gutierrez-Campos
    Juan Antonio Torres-Acosta
    Luis Jorge Saucedo-Arias
    Miguel Angel Gomez-Lim
    [J]. Nature Biotechnology, 1999, 17 : 1223 - 1226
  • [42] The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic tobacco plants
    Gutierrez-Campos, R
    Torres-Acosta, JA
    Saucedo-Arias, LJ
    Gomez-Lim, MA
    [J]. NATURE BIOTECHNOLOGY, 1999, 17 (12) : 1223 - 1226
  • [43] Exogenous electrical stimulation of tomato plants: Induction of proteinase inhibitor II gene expression and control of photosynthetic activity
    Herde, O
    Willmitzer, L
    Fisahn, J
    [J]. PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V, 1998, : 4179 - 4182
  • [44] Soybean Kunitz, C-II and PI-IV inhibitor genes confer different levels of insect resistance to tobacco and potato transgenic plants
    S. Marchetti
    M. Delledonne
    C. Fogher
    C. Chiabà
    F. Chiesa
    F. Savazzini
    A. Giordano
    [J]. Theoretical and Applied Genetics, 2000, 101 : 519 - 526
  • [45] Soybean Kunitz, C-II and PI-IV inhibitor genes confer different levels of insect resistance to tobacco and potato transgenic plants
    Marchetti, S
    Delledonne, M
    Fogher, C
    Chiabà, C
    Chiesa, F
    Savazzini, F
    Giordano, A
    [J]. THEORETICAL AND APPLIED GENETICS, 2000, 101 (04) : 519 - 526
  • [46] Molecular basis for the resistance of an insect chymotrypsin to a potato type II proteinase inhibitor
    Dunse, K. M.
    Kaas, Q.
    Guarino, R. F.
    Barton, P. A.
    Craik, D. J.
    Anderson, M. A.
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (34) : 15016 - 15021
  • [47] Development of insect-resistant transgenic plants using plant genes: Expression of cowpea trypsin inhibitor in transgenic tobacco plants
    Sane, VA
    Nath, P
    Aminuddin
    Sane, PV
    [J]. CURRENT SCIENCE, 1997, 72 (10): : 741 - 747
  • [48] Production of transgenic soybean plants overexpressing plant cysteine proteinase inhibitors to confer insect resistance
    Yamada, S
    Ueki, H
    Kita, Y
    Nishizawa, K
    Kuroda, M
    Yoza, K
    Kitayama, M
    Ishimoto, M
    [J]. PLANT AND CELL PHYSIOLOGY, 2005, 46 : S147 - S147
  • [49] TRANSGENIC TOBACCO PLANES WITH EFFICIENT INSECT RESISTANCE
    LI, TY
    TIAN, YC
    QIN, XF
    MANG, KQ
    LI, WG
    HE, YG
    SHEN, L
    [J]. SCIENCE IN CHINA SERIES B-CHEMISTRY LIFE SCIENCES & EARTH SCIENCES, 1994, 37 (12): : 1479 - 1488
  • [50] SIGNALS INVOLVED IN WOUND-INDUCED PROTEINASE-INHIBITOR-II GENE-EXPRESSION IN TOMATO AND POTATO PLANTS
    PENACORTES, H
    FISAHN, J
    WILLMITZER, L
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) : 4106 - 4113
12345