Transgenic tobacco plants harboring tomato proteinase inhibitor II gene and their insect resistance

被引：0
|
作者：
Xie, XZ
Huang, MJ
Wu, NH [1 ]
机构：
[1] Chinese Acad Sci, Inst Dev Biol, Beijing 100080, Peoples R China
[2] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
来源：
PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL | 2002年 / 12卷 / 03期
关键词：
tomato proteinase inhibitor II gene; transgenic tobacco; insect-resistance;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The plant expression vectors pBCT2 and pBT2 were constructed with the cDNA sequence ( tin2) and genomic DNA sequence (tin2i) of tomato proteinase inhibitor II gene respectively. Then the two expression vectors were transferred into tobacco via the Agrobacterium tumefaciens strain LBA4404, and transgenic tobacco plants were generated. Molecular analysis and trypsin activity assay showed that both cDNA and genomic DNA were expressed properly in the transgenic plants. Insecticidal activities in these transgenic plants indicated that transgenic tobacco plants carrying tin2i sequence were more resistant to 2-instar larvae of Heliothis armigera Hubner than those carrying tin2 sequence. Therefore the intron of tin2i sequence might be a contributor to insecticidal activity of the transgenic tobacco.
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页码：198 / 203
页数：6
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