Gene expression profiling in single cells within tissue

被引：40

作者：

Capodieci, P

Donovan, M

Buchinsky, H

Jeffers, Y

Cordon-Cardo, C

Gerald, W

Edelson, J

Shenoy, SM

Singer, RH

机构：

[1] Aureon Labs, Yonkers, NY 10701 USA

[2] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA

[3] Albert Einstein Coll Med, Bronx, NY 10461 USA

来源：

NATURE METHODS | 2005年 / 2卷 / 09期

关键词：

D O I：

10.1038/NMETH786

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.

引用

页码：663 / 665

页数：3

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