Gene expression profiling in single cells within tissue

被引：40
|
作者：
Capodieci, P
Donovan, M
Buchinsky, H
Jeffers, Y
Cordon-Cardo, C
Gerald, W
Edelson, J
Shenoy, SM
Singer, RH
机构：
[1] Aureon Labs, Yonkers, NY 10701 USA
[2] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA
[3] Albert Einstein Coll Med, Bronx, NY 10461 USA
来源：
NATURE METHODS | 2005年 / 2卷 / 09期
关键词：
D O I：
10.1038/NMETH786
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed a robust multiplex fluorescent in situ hybridization (FISH) technique in archival formalin-fixed, paraffin-embedded (FFPE) human tissue sections while preserving the microanatomical context. This identifies single-cell gene expression patterns by probing multiple, unique nascent RNA transcripts and yields predictive quantitative gene expression signatures.
引用
收藏
页码：663 / 665
页数：3
相关论文
共 50 条
  • [1] Gene expression profiling in single cells within tissue
    Capodieci P.
    Donovan M.
    Buchinsky H.
    Jeffers Y.
    Cordon-Cardo C.
    Gerald W.
    Edelson J.
    Shenoy S.M.
    Singer R.H.
    [J]. Nature Methods, 2005, 2 (9) : 663 - 665
  • [2] Gene expression profiling within a single mouse retina neuron
    Han, Y
    Massey, SC
    [J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 : U409 - U409
  • [3] Gene expression profiling of single Hodgkin lymphoma cells
    Mollweide, A.
    Richter, G. H. S.
    Kremer, M.
    Fend, F.
    Klein, C.
    Koerholz, D.
    Burdach, S.
    [J]. KLINISCHE PADIATRIE, 2007, 219 (03): : 189 - 189
  • [4] Gene expression profiling of single haematopoietic precursor cells
    Sakhinia, E
    Bashein, A
    Buckle, AM
    Byers, R
    Brady, G
    [J]. EXPERIMENTAL HEMATOLOGY, 2005, 33 (07) : 83 - 83
  • [5] Classification of Subpopulations of Cells Within Human Primary Brain Tumors by Single Cell Gene Expression Profiling
    Mollerstrom, Elin
    Rydenhag, Bertil
    Andersson, Daniel
    Lebkuechner, Isabell
    Puschmann, Till B.
    Chen, Meng
    Wilhelmsson, Ulrika
    Stahlberg, Anders
    Malmgren, Kristina
    Pekny, Milos
    [J]. NEUROCHEMICAL RESEARCH, 2015, 40 (02) : 336 - 352
  • [6] Classification of Subpopulations of Cells Within Human Primary Brain Tumors by Single Cell Gene Expression Profiling
    Elin Möllerström
    Bertil Rydenhag
    Daniel Andersson
    Isabell Lebkuechner
    Till B. Puschmann
    Meng Chen
    Ulrika Wilhelmsson
    Anders Ståhlberg
    Kristina Malmgren
    Milos Pekny
    [J]. Neurochemical Research, 2015, 40 : 336 - 352
  • [7] Simultaneous Profiling of Chromosome Conformation and Gene Expression in Single Cells
    Chen, Yujie
    Xu, Heming
    Liu, Zhiyuan
    Xing, Dong
    [J]. BIO-PROTOCOL, 2023, 13 (22):
  • [8] Comprehensive qPCR profiling of gene expression in single neuronal cells
    Citri, Ami
    Pang, Zhiping P.
    Suedhof, Thomas C.
    Wernig, Marius
    Malenka, Robert C.
    [J]. NATURE PROTOCOLS, 2012, 7 (01) : 118 - 127
  • [9] Simultaneous Profiling of Gene Expression and Chromatin Accessibility in Single Cells
    Reyes, Miguel
    Billman, Kianna
    Hacohen, Nir
    Blainey, Paul C.
    [J]. ADVANCED BIOSYSTEMS, 2019, 3 (11)
  • [10] TARGETED GENE EXPRESSION PROFILING WITHIN CIRCULATING TUMOR CELLS IN MCRPC
    Singhal, Udit
    Wang, Yugang
    Reichert, Zachary
    Tomlins, Scott
    Morgan, Todd
    [J]. JOURNAL OF UROLOGY, 2018, 199 (04): : E1188 - E1188
12345