Detection of cdtA, cdtB, and cdtC genes in Campylobacter jejuni by multiplex PCR

被引：52
|
作者：
Martínez, I [1 ]
Mateo, E [1 ]
Churruca, E [1 ]
Girbau, C [1 ]
Alonso, R [1 ]
Fernández-Astorga, A [1 ]
机构：
[1] Univ Pais Vasco UPV EHU, Fac Farm, Dept inmunol Microbiol & Parasitol, E-01006 Vitoria, Spain
来源：
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY | 2006年 / 296卷 / 01期
关键词：
cdtA; cdtB; cdtC; Campylobacter jejuni; multiplex PCR;
D O I：
10.1016/j.ijmm.2005.08.003
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A multiplex PCR was developed for simultaneous detection of the cytolethal distending toxin (cdt) genes of Campylobacter jejuni. Three primer pairs targeting each one of the cdtA, cdtB and cdtC genes were designed and combined in the same PCR reaction. The assay was evaluated with 100 C. jejuni strains recovered from humans and animals and it was found to be rapid and specific. Two isolates presented several deletions affecting both cdtA and cdtB genes. High prevalence (98%) of the three cdt genes was found among isolates of different geographic origins. (C) 2005 Elsevier GmbH. All rights reserved.
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收藏
页码：45 / 48
页数：4
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