Loop-Mediated Isothermal Amplification (LAMP) for Detection of Campylobacter jejuni and C. coli in Thai Children with Diarrhea

被引：16

作者：

Ngan Thi Kim Pham ^{[1
]}

Quang Duy Trinh ^{[1
]}

Khamrin, Pattara ^{[3
]}

Ukarapol, Nuthapong ^{[4
]}

Kongsricharoern, Tipachan ^{[5
]}

Yamazaki, Wataru ^{[2
]}

Komine-Aizawa, Shihoko ^{[1
]}

Okitsu, Shoko ^{[1
]}

Maneekarn, Niwat ^{[3
]}

Hayakawa, Satoshi ^{[1
]}

Ushijima, Hiroshi ^{[1
]}

机构：

[1] Nihon Univ, Sch Med, Dept Pathol & Microbiol, Div Microbiol, Tokyo 1738610, Japan

[2] Miyazaki Univ, Fac Agr, Dept Vet Sci, Miyazaki 8892192, Japan

[3] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai 50000, Thailand

[4] Chiang Mai Univ, Fac Med, Dept Pediat, Chiang Mai 50000, Thailand

[5] Nakornping Hosp, Pediat Hematol Unit, Chiang Mai, Thailand

来源：

JAPANESE JOURNAL OF INFECTIOUS DISEASES | 2015年 / 68卷 / 05期

基金：

日本学术振兴会;

关键词：

FOOD ANIMALS; SAMPLES; IDENTIFICATION; ASSAY; PCR;

D O I：

10.7883/yoken.JJID.2014.450

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Campylobacter species are common causes of bacterial diarrhea, and Campylobacter jejuni and C. coli are known as the predominant causative agents in humans. Recent studies suggested that loop-mediated isothermal amplification (LAMP) is an efficient and practical tool for rapid detection of C. jejuni and C. coli in clinical samples. We used LAMP to screen 151 stool samples for Campylobacter; these samples were collected in 2012 from Thai children with diarrhea. The PCR method discriminated C. jejuni and C. coli among the detected Campylobacter strains; these species were subjected to sequencing of the hip gene (in C. jejuni) or the ask gene (in C. coli). The results suggest that the prevalence of Campylobacter infection among Thai children with diarrhea is 8.6%, and C. jejuni is the most prevalent species.

引用

页码：432 / 433

页数：2

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