Characterisation and identification of arbuscular mycorrhizal fungi species by PCR/RFLP analysis of the rDNA internal transcribed spacer (ITS)

被引：0

作者：

do Espirito Santo Mergulhao, Adalia Cavalcanti ^{[1
]}

da Silva, Marcia Vanusa ^{[2
]}

Barreto Figueiredo, Marcia do Vale ^{[1
]}

Burity, Helio Almeida ^{[1
]}

Maia, Leonor Costa ^{[2
]}

机构：

[1] Inst Agron Pernanbuco IPA, Labs Genoma & Biol Solo, BR-50761000 Recife, PE, Brazil

[2] Univ Fed Pernambuco UFPE, Dept Bioquim & Micol, BR-50670420 Recife, PE, Brazil

来源：

ANNALS OF MICROBIOLOGY | 2008年 / 58卷 / 02期

关键词：

AM fungi; ITS; Glomus and Paraglomus species;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The polymerase chain reaction coupled with restriction fragment length polymorphism analysis (PCR/RFLP) was applied to distinguish arbuscular mycorrhizal fungi (AMF) species from an impacted semiarid soil. The ITS1-5.8S-ITS2 region was amplified with the primers ITS1 and ITS4 and the products of amplification were digested with the restriction enzymes HinfI, MboI and AluI. The obtained banding pattern, except for the AluI, allowed the distinction at the molecular level of the AMF species: Paraglomus occultum, Glomus mosseae, Glomus intraradices and Glomus etunicatum. The results showed that this technique has a potential to be used as a marker to differentiate AMF species with high phylogenetic affinity.

引用

页码：341 / 344

页数：4

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