DNA/AuNP Fluorescent Detecting Nano-Device

A DNA/AuNP (gold nanoparticle) detecting nano-device has been constructed using DNA displacement (three-way branch migration).Here, DNA/AuNP conjugations and DNA circle are utilized as basic parts in nano-system, while sensitive fluorescent signals are applied as output results. Because of the accurate molecular recognition and effective strand displacements, this detecting system is able to yield correct results after receiving specific input DNA signals. In this work, the results are detected at three levels: PAGE gel bands, fluorescent signals and TEM images. Especially, in TEM images, positions of AuNPs are detected as an alternative approach to obtain output results. Combing these multiple detecting methods together, advantages of this DNA/AuNP system could be taken used entirely. It is believed that DNA/AuNPs conjugations nano-device will be applied widely in molecular detecting fields.