Real-time control of a microfluidic channel for size-independent deformability cytometry

被引：25

作者：

Guan, Guofeng ^{[1
,2
]}

Chen, Peter C. Y. ^{[1
,2
]}

Peng, Weng Kung ^{[2
]}

Bhagat, Ali Asgar ^{[2
,3
]}

Ong, Chong Jin ^{[1
]}

Han, Jongyoon ^{[2
,4
,5
]}

机构：

[1] Natl Univ Singapore, Dept Mech Engn, Singapore 117548, Singapore

[2] Singapore MIT Alliance Res & Technol SMART, Biosyst & Micromech IRG, Singapore, Singapore

[3] Clearbridge BioMedics Pte Ltd, Singapore, Singapore

[4] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

[5] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA

来源：

JOURNAL OF MICROMECHANICS AND MICROENGINEERING | 2012年 / 22卷 / 10期

关键词：

LIVING CELLS; MEMBRANE; TWEEZERS;

D O I：

10.1088/0960-1317/22/10/105037

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Mechanical properties of cells can be correlated with various cell states and are now considered as an important class of biophysical markers. Effectiveness of existing high-throughput microfluidic techniques for investigating cell mechanical properties is adversely affected by cell-size variation in a given cell population. In this work, we introduce a new microfluidic system with real-time feedback control to evaluate single-cell deformability while minimizing cell-size dependence of the measurement. Using breast cancer cells (MCF-7), we demonstrate the potential of this system for stiffness profiling of cells in complex, diverse cell populations.

引用

页数：7

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