Polymerase chain reaction typing of genes of the locus of enterocyte effacement of ruminant attaching and effacing Escherichia coli

被引：0
|
作者：
Yuste, Maria [1 ]
Orden, Jose A. [1 ]
De La Fuente, Ricardo [1 ]
Ruiz-Santa-Quiteria, Jose A. [1 ]
Cid, Dolores [1 ]
Martinez-Pulgarin, Susana [1 ]
Dominguez-Bernal, Gustavo [1 ]
机构：
[1] Univ Complutense, Fac Vet, Dept Sanidad Anim, E-28040 Madrid, Spain
来源：
CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE | 2008年 / 72卷 / 05期
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D O I：
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中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The variability of the tir, espA, and espD genes of the locus of enterocyte effacement (LEE) in 185 attaching and effacing Escherichia coli (AEEC) strains isolated from healthy and diarrheic cattle, sheep, and goats was investigated by polymerase chain reaction. Nineteen of the strains were enterohemorrhagic E. coli (EHEC); the other 166 were enteropathogenic E. coli (EPEC). The combinations of the tir and esp genes were associated with the variants of the eae gene but not with a strain's belonging to the EPEC or EHEC group, animal species, or health status (healthy or diarrheic) of the animal. In addition, most of the strains showed the same combinations of LEE genes and serogroups as have been found in AEEC strains isolated from humans, which indicates that ruminants seem to be an EPEC reservoir for humans.
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页码：444 / 448
页数：5
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