Studying the Effective Concentration of RGDS on RGDS-Poly(DL-Lactic Acid)

RGDS-PDLLA is a biomimetic material obtained by covalently incorporating RGDS adhesion peptide. RGDS-PDLLA was prepared on basis of BDPLA (butanediamine-grafted polyactic acid). In present work, we prepared RGDS-PDLLA with gradient concentration RGDS and evaluated the cytocompatibility of gradient RGDS-PDLLA and took a novel method for studying the effective concentration of RGDS after grafting on BDPLA. When the concentration of RGDS in the RGDS-grafted-PDLLA (RGDS-PDLLA) varied from 0.142 to 0.302 mu mol/g, cell cytocompatibility of osteoblasts was altered depending on the concentration of RGDS. 0.1 mu mol/g RGDS on RGDS-PDLLA (RGDS-PDLLA) membrane can effectively promoted cell attachment, cell spreading. The concentration of RGDS increased from 0.142 to 0.302 mu mol/g, the adhesion of osteoblasts on RGDS-PDLLA membrane increased slightly. However, we can find that when the RGDS increased to 2.42 mu mol/g, the cell on films increased. These results suggest that the cell adhesion is sensitive to the RGDS concentration. By the study, we can confirm the optimal concentration of RGDS on RGDS-PDLLA.