Resistance to Biocides in Listeria monocytogenes Collected in Meat-Processing Environments

被引:37
|
作者
Conficoni, Daniele [1 ,2 ]
Losasso, Carmen [1 ]
Cortini, Enzo [1 ]
Di Cesare, Andrea [3 ]
Cibin, Veronica [1 ]
Giaccone, Valerio [2 ]
Corno, Gianluca [3 ]
Ricci, Antonia [1 ]
机构
[1] Ist Zooprofilatt Sperimentale Venezie, Dipartimento Sicurezza Alimentare, Padua, Italy
[2] Univ Padua, Dept Anim Med Prod & Hlth, Padua, Italy
[3] CNR, ISE, Microbial Ecol Grp, Verbania, Italy
来源
FRONTIERS IN MICROBIOLOGY | 2016年 / 7卷
关键词
Listeria monocytogenes; food-processing plants; environment; resistance genes; gene expression; efflux pumps; BENZALKONIUM CHLORIDE; GENE-EXPRESSION; EFFLUX PUMPS; HEAVY-METAL; SALMONELLA; TOLERANCE; INDUSTRY; PLANTS; COLI;
D O I
10.3389/fmicb.2016.01627
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The emergence of microorganisms exerting resistance to biocides is a challenge to meat-processing environments. Bacteria can be intrinsically resistant to biocides but resistance can also be acquired by adaptation to their sub-lethal concentrations. Moreover, the presence of biocide resistance determinants, which is closely linked to antibiotic resistance determinants, could lead to co-selection during disinfection practices along the food chain, and select cross-resistant foodborne pathogens. The purpose of this work was to test the resistance of wild strains of Listeria monocytogenes, isolated from pork meat processing plants, toward benzalkonium chloride (BC), used as proxy of quaternary ammonium compounds. Furthermore, the expression of two non-specific efflux pumps genes (Ide and mdrL) under biocide exposure was evaluated. L. monocytogenes were isolated from five processing plants located in the Veneto region (northeast of Italy) before and after cleaning and disinfection (C&D) procedures. A total of 45 strains were collected: 36 strains before and nine after the C&D procedures. Collected strains were typed according to MLST and ERIC profiles. Strains sampled in the same site, isolated before, and after the C&D procedures and displaying the same MLST and ERIC profiles were tested for their sensitivity to different concentrations of BC, in a time course assay. The expression of non-specific efflux pumps was evaluated at each time point by qPCR using tufA gene as housekeeping. A differential expression of the two investigated genes was observed: Ide was found to be more expressed by the strains isolated before C&D procedures while its expression was dose-dependent in the case of the post C&D procedures strain. On the contrary, the expression of mdrL was inhibited under low biocidal stress (10 ppm BC) and enhanced in the presence of high stress (100 ppm BC). These findings suggests a possible role for C&D procedures to select L. monocytogenes persisters, pointing out the importance of dealing with the identification of risk factors in food plants sanification procedures that might select more tolerant strains.
引用
收藏
页数:9
相关论文
共 50 条
  • [41] QUALITY-ASSURANCE IN MEAT-PROCESSING FACTORY
    HOGG, KJ
    FLEISCHWIRTSCHAFT, 1995, 75 (03): : 238 - &
  • [42] Genotyping of Listeria monocytogenes strains in Italian food-processing environments
    Torresi, M.
    Chiaverini, A.
    Acciari, V. A.
    Centorame, P.
    Centorotola, G.
    Guidi, F.
    Russini, V.
    Pomilio, F.
    EUROPEAN JOURNAL OF PUBLIC HEALTH, 2021, 31 : 575 - 575
  • [43] Investigation of Listeria monocytogenes in workers, equipment and environments at Kaymak processing plants
    Kara, Recep
    Aslan, Savas
    FOOD SCIENCE AND TECHNOLOGY, 2021, 41 (02): : 449 - 452
  • [44] The incidence of Listeria monocytogenes in raw meat
    Yucel, N
    Citak, S
    Gundogan, N
    INDIAN VETERINARY JOURNAL, 2004, 81 (11): : 1192 - 1194
  • [45] Radioresistance of Listeria monocytogenes in meat and cheese
    Szczawinski, J
    Szczawinska, ME
    Stanczak, B
    ACTA ALIMENTARIA, 1996, 25 (04) : 397 - 401
  • [46] Prevalence of Listeria monocytogenes in poultry meat
    Elmali, Mehmet
    Can, Hayriye Yesim
    Yaman, Hilmi
    FOOD SCIENCE AND TECHNOLOGY, 2015, 35 (04): : 672 - 675
  • [47] High-Pressure Processing Influences Antibiotic Resistance Gene Transfer in Listeria monocytogenes Isolated from Food and Processing Environments
    Wisniewski, Patryk
    Chajecka-Wierzchowska, Wioleta
    Zadernowska, Anna
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (23)
  • [48] MEAT-PROCESSING, FISH PROCESSING, AND POULTRY-PROCESSING WASTES
    LITCHFIELD, JH
    JOURNAL WATER POLLUTION CONTROL FEDERATION, 1981, 53 (06): : 787 - 791
  • [49] Genomic persistence of Listeria monocytogenes strains in dairy and meat processing plants in Italy
    Acciari, V. A.
    Del Matto, I.
    Antoci, S.
    Di Marzio, V.
    Torresi, M.
    Iannetti, L.
    Marfoglia, C.
    Centorotola, G.
    Pomilio, F.
    EUROPEAN JOURNAL OF PUBLIC HEALTH, 2021, 31
  • [50] Dynamics of Listeria monocytogenes colonisation in a newly-opened meat processing facility
    Bolocan, Andrei Sorin
    Nicolau, Anca Ioana
    Alvarez-Ordonez, Avelino
    Borda, Daniela
    Oniciuc, Elena Alexandra
    Stessl, Beatrix
    Gurgu, Leontina
    Wagner, Martin
    Jordan, Kieran
    MEAT SCIENCE, 2016, 113 : 26 - 34