Stopped-flow kinetic fluorimetric studies of the interaction of Ru(II) complex with DNA and its analytical application

A simple, rapid, and sensitive stopped-now kinetic fluorometric approach was established for the assay of DNA in synthetic samples and real samples by using the measures of initial reaction rate. The increased initial reaction rate is in proportion to the concentration of DNA in the range of 2.0 x 10(-8) M to 2.1 x 10(-6) M. The optimum conditions for various parameters on which the binding of Ru(phen)(2)(dppz)(2+) to DNA depends, were investigated. The influence of various surfactants on the interaction was discussed. Furthermore, stopped-flow techniques were employed to determine kinetic parameters of Ru(phen)(2)(dppZ)(2+) binding to DNA under pseudo-first-order conditions. It was found that the interaction of Ru(phen)(2)(dppZ)(2+) with DNA was very fast. A two-step reaction mechanism, a fast phase followed by a slow phase, was proposed. (C) 2008 Elsevier B.V. All rights reserved.