Measurement of nanomechanical properties of DNA molecules by PeakForce atomic force microscopy based on DNA origami

被引：12

作者：

Li, Lin ^{[1
]}

Zhang, Ping ^{[2
,3
,4
]}

Li, Jiang ^{[2
,3
,5
]}

Wang, Ying ^{[2
,3
,5
]}

Wei, Yuhui ^{[2
,3
,5
]}

Hu, Jun ^{[2
,3
,5
]}

Zhou, Xingfei ^{[1
,6
]}

Xu, Bingqian ^{[6
]}

Li, Bin ^{[2
,3
,5
]}

机构：

[1] Ningbo Univ, Sch Sci, Ningbo 315211, Zhejiang, Peoples R China

[2] Chinese Acad Sci, Div Phys Biol, Shanghai 201800, Peoples R China

[3] Chinese Acad Sci, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, Key Lab Interfacial Phys & Technol,Shanghai Inst, Shanghai 201800, Peoples R China

[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[5] Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China

[6] Univ Georgia, Coll Engn, Single Mol Study Lab, Athens, GA 30602 USA

来源：

NANOSCALE | 2019年 / 11卷 / 11期

基金：

中国国家自然科学基金;

关键词：

ELASTIC-MODULI; MECHANICAL-PROPERTIES; SINGLE-MOLECULE; NANOSCALE; PROTEIN; SOFT; FLEXIBILITY; RESOLUTION; TENSION; SURFACE;

D O I：

10.1039/c8nr10354b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Characterization of the stiffness of thin DNA strands remains difficult. By constructing bilayer DNA molecules, we investigated their mechanical properties using AFM. Increased DNA thickness through DNA origami greatly reduced the substrate effect when measuring Young's modulus, thus providing a more accurate picture of the inherent nanomechanical properties of DNA.

引用

页码：4707 / 4711

页数：5

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