Fabrication and characterization of a surface plasmon resonance based fiber-optic sensor for bittering component - Naringin

被引:24
|
作者
Rajan
Chand, S
Gupta, BD
机构
[1] Indian Inst Technol, Dept Phys, New Delhi 110016, India
[2] Indian Inst Technol, Dept Biochem Engn & Biotechnol, New Delhi 110016, India
关键词
surface plasmon resonance (SPR); immobilized enzymes; biosensors; sensitivity; signal to noise ratio (SNR);
D O I
10.1016/j.snb.2005.09.023
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The fabrication and characterization of a surface plasmon resonance (SPR) based fiber-optic sensor to determine the amount of naringin, which causes bittering effect in citrus fruit juices, have been reported. The sensing region has been fabricated by coating about I cm length of the unclad core with silver. To sense naringin, the enzyme, naringinase, has been immobilized over the metallic surface. The spectral interrogation method has been used to characterize the sensor. It has been observed that the SPR wavelength increases with an increase in the concentration of naringin. In addition, the sensitivity, reproducibility and the signal to noise ratio (SNR) of the sensor have been studied. It has been found that the sensitivity increases with an increase in the concentration of naringin, while the reverse is the case for SNR. The fiber-optic SPR sensing probe reported can be repeatedly used. Further, it can be used for remote and in situ monitoring of the naringin concentration. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:344 / 348
页数:5
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