Effect of Plant Growth Regulators on Tissue Culture in Chinese Jujube

Plant growth regulator is indispensable material in culture media, and is important to the plant tissue induction, organ differentiation and growth. Therefore, the effects of seven plant growth regulators such as BA, TDZ, KT, NAA, IBA, IAA and GA(3) with different concentrations on the leaf regeneration, subculture of shoots and rooting were studied in Ziziphus jujuba cv. 'Dongzao'. The results indicated that the efficiency of TDZ was significantly higher than that of BA in the induction of adventitious bud from leaf. Leaves should be first induced on MS medium supplemented with TDZ (1.0 mg/L) and IBA (0.1 mg/L) for 28 days, and then transferred to medium MS+IBA 0.1 mg/L+GA(3) 0.05 mg/L. In this way, the regeneration rate reached 92.45%. MS+BA 1.0 mg/L+KT 0.5 mg/L+IBA 0.1 mg/L was suitable for subculture of shoots, with the multiplication coefficient of 3.64. Supplements of GA(3) at 0.5 mg/L in the medium could significantly increase the elongation of shoots. The efficiency of IAA was best among that of IAA, IBA and NAA in the induction of rooting. The regenerated plantlets rooted well in 1/2MS medium plus IAA (1.5 mg/L), with rooting percentage of 95.3%.