Non-homologous end-joining proteins are required for Agrobacterium T-DNA integration

被引:102
|
作者
van Attikum, H [1 ]
Bundock, P [1 ]
Hooykaas, PJJ [1 ]
机构
[1] Leiden Univ, Clusius Lab, Inst Mol Plant Sci, NL-2333 AL Leiden, Netherlands
来源
EMBO JOURNAL | 2001年 / 20卷 / 22期
关键词
Agrobacterium; genomic instability; non-homologous end-joining; T-DNA integration; telomeres;
D O I
10.1093/emboj/20.22.6550
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Agrobacterium tumefaciens causes crown gall disease in dicotyledonous plants by introducing a segment of DNA (T-DNA), derived from its tumour-inducing (Ti) plasmid, into plant cells at infection sites. Besides these natural hosts, Agrobacterium can deliver the T-DNA also to monocotyledonous plants, yeasts and fungi. The T-DNA integrates randomly into one of the chromosomes of the eukaryotic host by an unknown process. Here, we have used the yeast Saccharomyces cerevisiae as a T-DNA recipient to demonstrate that the non-homologous end-joining (NHEJ) proteins Yku70, Rad50, Mre11, Xrs2, Lig4 and Sir4 are required for the integration of T-DNA into the host genome. We discovered a minor pathway for T-DNA integration at the telomeric regions, which is still operational in the absence of Rad50, Mre11 or Xrs2, but not in the absence of Yku70. T-DNA integration at the telomeric regions in the rad50, mre11 and xrs2 mutants was accompanied by gross chromosomal rearrangements.
引用
收藏
页码:6550 / 6558
页数:9
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