Imaging of RNAs in live cells with spectrally diverse small molecule fluorophores

被引：11
|
作者：
Carrocci, Tucker J. [1 ]
Hoskins, Aaron A. [1 ]
机构：
[1] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA
来源：
ANALYST | 2014年 / 139卷 / 01期
关键词：
LIVING CELLS; IN-VIVO; PROTEIN; YEAST; TAG;
D O I：
10.1039/c3an01550e
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have developed new protein-based tags for use in imaging of RNAs in living cells. These tags are based on the MS2 phage coat protein fused to either E. coli dihydrofolate reductase or the SNAP tag. Labeled RNAs can be visualized with fluorophores spanning the visible spectra range.
引用
收藏
页码：44 / 47
页数：4
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