Chemical interactions of mercury species and some transition and noble metals towards metallothionein (Zn7MT-2) evaluated using SEC/ICP-MS, RP-HPLC/ESI-MS and MALDI-TOF-MS

被引：14
|
作者：
Xu, Ming [1 ,2 ]
Yang, Limin [1 ,2 ]
Wang, Qiuquan [1 ,2 ,3 ]
机构：
[1] Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China
[2] Xiamen Univ, Key Lab Analyt Sci, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China
[3] Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China
来源：
METALLOMICS | 2013年 / 5卷 / 07期
基金：
中国国家自然科学基金;
关键词：
MASS-SPECTROMETRY; ICP-MS; BINDING; IDENTIFICATION; PROTEINS; MECHANISM; TOXICITY; PEPTIDE; LIVER; MICE;
D O I：
10.1039/c3mt00016h
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The chemical affinities of three submetallomes ("Mn, Co, Ni, Cu, Cd''; "Pd, Pt, Au''; and "Hg, CH3Hg, C2H5Hg-THI'') towards metallothionein-2 (Zn7MT-2) in a physiological solution environment were evaluated using SEC/ICP-MS together with RP-HPLC/ESI-IT-MS and MALDI-TOF-MS, and followed the order: "Zn2+ < Cu2+, Cd2+ > Ni2+ > Mn2+, Co2+''; "Pd2+ > Au3+ > Pt2+''; and "Hg2+ > CH3Hg+ > C2H5Hg-THI''. Besides these, the structural change and composition of the CH3Hg-MT-2 complexes formed during the CH3Hg+ titration process were further investigated using CD spectroscopy, RP-HPLC/ESI-MS and MALDI-TOF-MS, indicating that linear and more hydrophobic (CH3Hg)(x)MT-2 (x = 12, 13, 14, 15, 16, 17, 18, 19 and 20) were formed. This information is important in understanding the interactions of the metals with Zn7MT-2 and their corresponding biological functions and toxicities.
引用
收藏
页码：855 / 860
页数：6
相关论文
共 2 条
  • [1] Joint forces of mass spectrometric techniques (ICP-MS and MALDI-TOF-MS) and fluorescence spectrometry in the study of platinum-based cytostatic drugs interactions with metallothionein MT2 and MT3
    Pavelicova, Kristyna
    Do, Tomas
    Vejvodova, Marketa
    Vaculovic, Tomas
    Nowak, Kinga
    Matczuk, Magdalena
    Wu, Sylwia
    Krężel, Artur
    Adam, Vojtech
    Vaculovicova, Marketa
    Talanta, 2024, 274
  • [2] Joint forces of mass spectrometric techniques (ICP-MS and MALDI-TOF-MS) and fluorescence spectrometry in the study of platinum-based cytostatic drugs interactions with metallothionein MT2 and MT3
    Pavelicova, Kristyna
    Do, Tomas
    Vejvodova, Marketa
    Vaculovic, Tomas
    Nowak, Kinga
    Matczuk, Magdalena
    Wu, Sylwia
    Krezel, Artur
    Adam, Vojtech
    Vaculovicova, Marketa
    TALANTA, 2024, 274
1