A genetic linkage map of crested wheatgrass based on AFLP and RAPD markers

被引：2

作者：

Yu, Xiaoxia ^{[1
,2
]}

Li, Xiaolei ^{[1
]}

Ma, Yanhong ^{[1
]}

Yu, Zhuo ^{[1
]}

Li, Zaozhe ^{[1
]}

机构：

[1] Inner Mongolia Agr Univ, Coll Agron, Hohhot 010019, Peoples R China

[2] China Agr Univ, Key Lab Crop Genom & Genet Improvement, Minist Agr, Beijing 100193, Peoples R China

来源：

GENOME | 2012年 / 55卷 / 04期

基金：

中国国家自然科学基金;

关键词：

crested wheatgrass; F-2; population; AFLP; RAPD; genetic linkage map; GROWTH HABIT; AGROPYRON-CRISTATUM; LEYMUS TRITICEAE; SSR; QTLS; F-2; POPULATIONS; INTEGRATION; L;

D O I：

10.1139/G2012-014

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Using a population of 105 interspecific F-2 hybrids derived from a cross between Agropyron mongolicum Keng and Agropyron cristatum (L.) Gaertn. 'Fairway' as a mapping population, a genetic linkage map of crested wheatgrass was constructed based on AFLP and RAPD molecular markers. A total of 175 markers, including 152 AFLP and 23 RAPD markers, were ordered in seven linkage groups. The map distance was 416 cM, with a mean distance of 2.47 cM between markers. The number of markers ranged from 13 to 46 in each linkage group and the length of groups ranged from 18 to 104 cM. The research found that 30 out of 175 molecular markers showed segregation distortion, accounting for 17% of all markers. This is the first genetic linkage map of crested wheatgrass. This map will facilitate gene localization, cloning, and molecular marker-assisted selection in the future.

引用

页码：327 / 335

页数：9

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