KCNQ-encoded channels regulate Na+ transport across H441 lung epithelial cells

被引：13
|
作者：
Greenwood, I. A. [1 ]
Yeung, S. Y. M. [1 ]
Hettiarachi, S. [1 ]
Andersson, M. [1 ]
Baines, D. L. [1 ]
机构：
[1] Univ London, Div Basic Med Sci, London SW17 0RE, England
来源：
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 2009年 / 457卷 / 04期
基金：
英国生物技术与生命科学研究理事会;
关键词：
K+ channel; KCNQ; Na+ transport; Epithelia; Lung; ACTIVATED PROTEIN-KINASE; BASOLATERAL K+ CHANNELS; POTASSIUM CHANNEL; CHLORIDE SECRETION; AIRWAY EPITHELIA; ANION SECRETION; CL-SECRETION; LINE CALU-3; II CELLS; EXPRESSION;
D O I：
10.1007/s00424-008-0557-7
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
H441 cells are a model of absorptive airway epithelia that are characterised by a pronounced apical Na+ flux through amiloride-sensitive Na+ channels. The flux of Na+ is intimately linked to Na+ handling by the cell as well as the membrane potential across the apical membrane. As KCNQ-encoded K+ channels influence chloride secretion in gastrointestinal epithelia, the goal of the present study was to ascertain the expression of KCNQ genes in H441 cells and determine the functional role of the expression products. Message for KCNQ3 and KCNQ5 was detected by RT-polymerase chain reaction and the translated proteins were observed by immunocytochemistry. Ussing experiments showed that the pan-KCNQ channel blocker XE991, but not KCNQ1 selective blockers, reduced the short circuit current and the amiloride-sensitive component. These data show for the first time that potassium channels encoded by KCNQ3 or KCNQ5 are crucial determinants of epithelial Na+ flux.
引用
收藏
页码：785 / 794
页数：10
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